Decoding Arabidopsis thaliana CPK/SnRK Superfamily Kinase Client Signaling Networks Using Peptide Library and Mass Spectrometry
Abstract
:1. Introduction
2. Materials and Methods
2.1. Synthesis and Development of the 2.1K Peptide Library
2.2. Preparation of Recombinant Protein Kinases
2.3. Purity and Activity Assessment of Recombinant Kinases
2.4. The Kinase Client Assay
2.5. LC-MS/MS Analysis
2.6. Database Search
2.7. Motif Analysis
2.8. Identification of Kinase Client Network
3. Results
3.1. Mass Spectrometry-Based Kinase Activity Confirmation of the Recombinant Kinases
3.2. Identification of Putative Clients for CDPKs, SnRKs, and PPCKs
3.3. Reconstruction of In Silico Kinase Client Signaling Networks
3.4. Cross-Validation of the CPK Clients
3.5. Identification of Kinase-Specific Motifs
4. Discussion
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Ahsan, N.; Kataya, A.R.A.; Rao, R.S.P.; Swatek, K.N.; Wilson, R.S.; Meyer, L.J.; Tovar-Mendez, A.; Stevenson, S.; Maszkowska, J.; Dobrowolska, G.; et al. Decoding Arabidopsis thaliana CPK/SnRK Superfamily Kinase Client Signaling Networks Using Peptide Library and Mass Spectrometry. Plants 2024, 13, 1481. https://doi.org/10.3390/plants13111481
Ahsan N, Kataya ARA, Rao RSP, Swatek KN, Wilson RS, Meyer LJ, Tovar-Mendez A, Stevenson S, Maszkowska J, Dobrowolska G, et al. Decoding Arabidopsis thaliana CPK/SnRK Superfamily Kinase Client Signaling Networks Using Peptide Library and Mass Spectrometry. Plants. 2024; 13(11):1481. https://doi.org/10.3390/plants13111481
Chicago/Turabian StyleAhsan, Nagib, Amr R. A. Kataya, R. Shyama Prasad Rao, Kirby N. Swatek, Rashaun S. Wilson, Louis J. Meyer, Alejandro Tovar-Mendez, Severin Stevenson, Justyna Maszkowska, Grazyna Dobrowolska, and et al. 2024. "Decoding Arabidopsis thaliana CPK/SnRK Superfamily Kinase Client Signaling Networks Using Peptide Library and Mass Spectrometry" Plants 13, no. 11: 1481. https://doi.org/10.3390/plants13111481