Accumulation of TIP2;2 Aquaporin during Dark Adaptation Is Partially PhyA Dependent in Roots of Arabidopsis Seedlings
Abstract
:1. Introduction
2. Results and Discussion
2.1. Effects of phyA Mutation on Fluorescent Intensity of TIP2;2-GFP under Light Conditions
2.1.1. Tissue-Specific Localization of TIP2;2-GFP in the Wild Type and phyA Mutant
2.1.2. Fluorescence of TIP2;2-GFP in Light-Grown Wild-Type and phyA Mutant Plants
F2 plant | Number of plants classified with phyA mutation and fluorescent intensity *1 | χ2 values *2 Expected frequency | |||||
---|---|---|---|---|---|---|---|
PHYA | phyA | ||||||
++ | + | − | ++ | + | − | ||
TIP2;2-GFP/ phyA 8-4 × Ler | 0 | 37 | 18 | 2 | 14 | 2 | 0.00456 [PHYA]: [phyA] = 3:1 1.54 [PHYA or GFP+]: [phyA gfp−] = 15:1 |
TIP2;2-GFP/ phyA 8-4 ×phyA | 0 | 0 | 0 | 2 | 18 | 3 | 1.75 [GFP+]:[gfp−] = 3:1 |
2.2. Dark-Induced Increase in TIP2;2-GFP
2.3. PhyA Regulation of Aquaporins
2.4. Why TIP2;2 Increases during Dark Adaptation
3. Experimental
3.1. Plant Material and Growth Conditions
3.2. Fluorescent Intensity Analysis
3.3. Protein Preparation
3.4. Immunoblotting
3.5. Generation of TIP2;2-GFP Transgenic Lines
3.6. Protoplast Preparation
3.7. RT-PCR
4. Conclusions
Supplementary Files
Supplementary File 1Acknowledgments
Author Contributions
Conflicts of Interest
References
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Uenishi, Y.; Nakabayashi, Y.; Tsuchihira, A.; Takusagawa, M.; Hashimoto, K.; Maeshima, M.; Sato-Nara, K. Accumulation of TIP2;2 Aquaporin during Dark Adaptation Is Partially PhyA Dependent in Roots of Arabidopsis Seedlings. Plants 2014, 3, 177-195. https://doi.org/10.3390/plants3010177
Uenishi Y, Nakabayashi Y, Tsuchihira A, Takusagawa M, Hashimoto K, Maeshima M, Sato-Nara K. Accumulation of TIP2;2 Aquaporin during Dark Adaptation Is Partially PhyA Dependent in Roots of Arabidopsis Seedlings. Plants. 2014; 3(1):177-195. https://doi.org/10.3390/plants3010177
Chicago/Turabian StyleUenishi, Yumi, Yukari Nakabayashi, Ayako Tsuchihira, Mari Takusagawa, Kayo Hashimoto, Masayoshi Maeshima, and Kumi Sato-Nara. 2014. "Accumulation of TIP2;2 Aquaporin during Dark Adaptation Is Partially PhyA Dependent in Roots of Arabidopsis Seedlings" Plants 3, no. 1: 177-195. https://doi.org/10.3390/plants3010177