Non-cultivated Cotton Species (Gossypium spp.) Act as a Reservoir for Cotton Leaf Curl Begomoviruses and Associated Satellites
Abstract
:1. Introduction
2. Results
2.1. Assembly and Analysis of NGS Data Identified Begomovirus and Alpha/Betasatellites in G. mustelinum, G. raimondii and G. thurberi
2.2. Diverse Species and Strains of Begomovirus/Alphasatellite/Betasatellite are Maintained by Non-cultivated Cotton Species
2.3. Begomovirus/Satellites are Phylogenetically Related to the Recent Cotton Leaf Curl Disease Complex
2.4. Begomovirus Levels are Reduced in the Presence of an Alphasatellite
3. Discussion
4. Materials and Methods
4.1. Sample Collection, DNA Extraction and Rolling Circle Amplification
4.2. Library Preparation, Sequencing, Assembly and Analysis
4.3. Alignment, SDT and Phylogeny
4.4. Recombination Analysis
4.5. Quantification with qPCR
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Species | Begomovirus/alphasatellite/betasatellite | Genomic Component | Strain |
---|---|---|---|
G. mustelinum (AADD) | Cotton leaf curl Multan virus | Monopartite | Pakistan |
Bhendi yellow vein mosaic virus | Monopartite | Thanagan | |
Cotton leaf curl Alabad virus | DNA-A | Multan | |
Cotton leaf curl Alabad virus | DNA-B | Multan | |
Cotton leaf curl Burewala alphasatellite | Alphasatellite | N. A | |
Ageratum yellow vein Singapore alphasatellite | Alphasatellite | N. A | |
Gossypium darwinii symptomless alphasatellite | Alphasatellite | N. A | |
Gossypium davidsonii symptomless alphasatellite | Alphasatellite | N. A | |
Papaya leaf curl alphasatellite | Alphasatellite | N. A | |
G. raimondii (DD) | Bhendi yellow vein mosaic virus | Monopartite | Thanagan |
Cotton leaf curl Alabad virus | DNA-A | Multan | |
Cotton leaf curl Alabad virus | DNA-B | Multan | |
Cotton leaf curl Multan virus | Monopartite | Pakistan | |
G. thurberi (DD) | Bhendi yellow vein mosaic virus | Monopartite | Pakistan |
Cotton leaf curl Alabad virus | DNA-A | Multan | |
Cotton leaf curl Alabad virus | DNA-B | Multan | |
Cotton leaf curl Alabad virus | DNA-A | Lobatum | |
Cotton leaf curl Alabad virus | DNA-B | Lobatum | |
Cotton leaf curl Multan virus | Monopartite | Pakistan | |
Cotton leaf curl Multan betasatellite | Betasatellite | Shahdadpur | |
Cotton leaf curl Multan betasatellite | Betasatellite | Burewala | |
Okra leaf curl betasatellite | Betasatellite | N. A |
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Shakir, S.; Zaidi, S.S.-e.-A.; Atiq-ur-Rehman; Farooq, M.; Amin, I.; Scheffler, J.; Scheffler, B.; Shah Nawaz-ul-Rehman, M.; Mansoor, S. Non-cultivated Cotton Species (Gossypium spp.) Act as a Reservoir for Cotton Leaf Curl Begomoviruses and Associated Satellites. Plants 2019, 8, 127. https://doi.org/10.3390/plants8050127
Shakir S, Zaidi SS-e-A, Atiq-ur-Rehman, Farooq M, Amin I, Scheffler J, Scheffler B, Shah Nawaz-ul-Rehman M, Mansoor S. Non-cultivated Cotton Species (Gossypium spp.) Act as a Reservoir for Cotton Leaf Curl Begomoviruses and Associated Satellites. Plants. 2019; 8(5):127. https://doi.org/10.3390/plants8050127
Chicago/Turabian StyleShakir, Sara, Syed Shan-e-Ali Zaidi, Atiq-ur-Rehman, Muhammad Farooq, Imran Amin, Jodi Scheffler, Brian Scheffler, Muhammad Shah Nawaz-ul-Rehman, and Shahid Mansoor. 2019. "Non-cultivated Cotton Species (Gossypium spp.) Act as a Reservoir for Cotton Leaf Curl Begomoviruses and Associated Satellites" Plants 8, no. 5: 127. https://doi.org/10.3390/plants8050127