Interspaced Repeat Sequences Confer the Regulatory Functions of AtXTH10, Important for Root Growth in Arabidopsis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Identification of Arabidopsis IRS and Functional Annotation of the IRS-Related Genes
2.2. Functional Identification of the IRSs in the CR6 and CR12 Promoters
2.3. Vector Construction and Subcellular Localization of the AtXTH10 Protein
2.4. Obtaining Transgenic AtXTH10 and Mutant Plants
2.5. Scanning Electron Microscopic Observations
2.6. Detection the Relative Expression Levels of Known Root Development-Related Genes by Reverse Transcription Quantitative Real-Time Polymerase Chain Reaction (RT-qPCR) in the Col, AtXTH10m and AtXTH10-OE Plants
3. Results and Discussion
3.1. Identification of IRSs and Functional Annotation of the IRS-Related Genes
3.2. IRSs Confer a Regulatory Function of Gene Expression
3.3. The AtXTH10 Gene Regulates the Growth and Development of Roots
3.4. Prediction of the Root Development Signaling Pathway Involved with the AtXTH10 Gene
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Cao, J.; Lv, Y.; Li, X. Interspaced Repeat Sequences Confer the Regulatory Functions of AtXTH10, Important for Root Growth in Arabidopsis. Plants 2019, 8, 130. https://doi.org/10.3390/plants8050130
Cao J, Lv Y, Li X. Interspaced Repeat Sequences Confer the Regulatory Functions of AtXTH10, Important for Root Growth in Arabidopsis. Plants. 2019; 8(5):130. https://doi.org/10.3390/plants8050130
Chicago/Turabian StyleCao, Jun, Yueqing Lv, and Xiang Li. 2019. "Interspaced Repeat Sequences Confer the Regulatory Functions of AtXTH10, Important for Root Growth in Arabidopsis" Plants 8, no. 5: 130. https://doi.org/10.3390/plants8050130