Overexpression of a Cytosolic 6-Phosphogluconate Dehydrogenase Gene Enhances the Resistance of Rice to Nilaparvata lugens
Abstract
:1. Introduction
2. Results
2.1. cDNA Cloning and Phylogenetic Analysis of the Os6PGDH1 Sequence
2.2. Expression of Os6PGDH1
2.3. Overexpressing of Os6PGDH1
2.4. Overexpression of Os6PGDH1 Enhances Levels of BPH-Induced JA, JA-Ile, and H2O2, but Suppresses Ethylene
2.5. Os6PGDH1 Positively Regulates the Resistance of Rice to BPH
3. Discussion
4. Materials and Methods
4.1. Plant Growth
4.2. Insects
4.3. Isolation and Characterization of Os6PGDH1
4.4. Sequence and Phylogenetic Analysis of Os6PGDH1
4.5. Generation and Characterization of Transgenic Plants
4.6. Plant Treatments
4.7. Quantitative Real-Time PCR
4.8. Os6PGDH1 mRNA Expression Analysis
4.9. Measurement of Plant Growth Parameters
4.10. JA, JA-Ile, and SA Analysis
4.11. Hydrogen Oxide Analysis
4.12. Ethylene Analysis
4.13. BPH Bioassays
4.14. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Chen, L.; Kuai, P.; Ye, M.; Zhou, S.; Lu, J.; Lou, Y. Overexpression of a Cytosolic 6-Phosphogluconate Dehydrogenase Gene Enhances the Resistance of Rice to Nilaparvata lugens. Plants 2020, 9, 1529. https://doi.org/10.3390/plants9111529
Chen L, Kuai P, Ye M, Zhou S, Lu J, Lou Y. Overexpression of a Cytosolic 6-Phosphogluconate Dehydrogenase Gene Enhances the Resistance of Rice to Nilaparvata lugens. Plants. 2020; 9(11):1529. https://doi.org/10.3390/plants9111529
Chicago/Turabian StyleChen, Lin, Peng Kuai, Miaofen Ye, Shuxing Zhou, Jing Lu, and Yonggen Lou. 2020. "Overexpression of a Cytosolic 6-Phosphogluconate Dehydrogenase Gene Enhances the Resistance of Rice to Nilaparvata lugens" Plants 9, no. 11: 1529. https://doi.org/10.3390/plants9111529