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Open AccessArticle
Trehalose Rescues Postmenopausal Osteoporosis Induced by Ovariectomy through Alleviating Osteoblast Pyroptosis via Promoting Autophagy
by
Xinli Hu
Xinli Hu 1,2,*,†,
Wei Wang
Wei Wang 1,2,†,
Xiaolong Chen
Xiaolong Chen 1,2,
Chao Kong
Chao Kong 1,2,
Xuan Zhao
Xuan Zhao 1,2,
Zheng Wang
Zheng Wang 1,2,‡,
Haojie Zhang
Haojie Zhang 1,2,‡ and
Shibao Lu
Shibao Lu 1,2,‡
1
Department of Orthopedics, Xuanwu Hospital, Capital Medical University, No. 45 Changchun Street, Xicheng District, Beijing 100053, China
2
National Clinical Research Center for Geriatric Diseases, Xuanwu Hospital, Capital Medical University, No. 45 Changchun Street, Xicheng District, Beijing 100053, China
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
‡
These authors also contributed equally to this work.
Biomedicines 2024, 12(10), 2224; https://doi.org/10.3390/biomedicines12102224 (registering DOI)
Submission received: 20 August 2024
/
Revised: 16 September 2024
/
Accepted: 27 September 2024
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Published: 29 September 2024
Abstract
Background: Osteoporosis, a prevalent bone metabolic disease, often requires long-term drug treatments that may lead to serious side effects. Trehalose, a natural disaccharide found in various organisms, has been shown to have a promoting effect on autophagy. However, whether trehalose can improve bone mass recovery in ovariectomized rats and its underlying mechanisms remains unclear. In this study, trehalose was administered to ovariectomized rats to evaluate its therapeutic potential for osteoporosis following ovariectomy. Methods: Micro-computed tomography (Micro-CT), hematoxylin and eosin (HE) and immunohistochemical staining techniques were utilized to evaluate the impact of trehalose on osteoporosis induced by ovariectomy (OVX) in mice, both in imaging and histological dimensions. Furthermore, the influence of trehalose on osteoblastogenesis and functional activity was quantified through Alizarin Red S (ARS) staining and immunoblotting assays. Results: Trehalose effectively mitigated bone loss, elevated autophagy and suppressed pyroptosis in ovariectomized rats. Furthermore, 3-methyladenine diminished the protective effects of trehalose, particularly in promoting autophagy and inhibiting pyroptosis. Conclusions: Trehalose demonstrates significant potential in treating osteoporosis by suppressing NLRP3 inflammasome-driven pyroptosis, primarily through autophagy promotion. This suggests that trehalose could be a promising, safer alternative treatment for osteoporosis.
Share and Cite
MDPI and ACS Style
Hu, X.; Wang, W.; Chen, X.; Kong, C.; Zhao, X.; Wang, Z.; Zhang, H.; Lu, S.
Trehalose Rescues Postmenopausal Osteoporosis Induced by Ovariectomy through Alleviating Osteoblast Pyroptosis via Promoting Autophagy. Biomedicines 2024, 12, 2224.
https://doi.org/10.3390/biomedicines12102224
AMA Style
Hu X, Wang W, Chen X, Kong C, Zhao X, Wang Z, Zhang H, Lu S.
Trehalose Rescues Postmenopausal Osteoporosis Induced by Ovariectomy through Alleviating Osteoblast Pyroptosis via Promoting Autophagy. Biomedicines. 2024; 12(10):2224.
https://doi.org/10.3390/biomedicines12102224
Chicago/Turabian Style
Hu, Xinli, Wei Wang, Xiaolong Chen, Chao Kong, Xuan Zhao, Zheng Wang, Haojie Zhang, and Shibao Lu.
2024. "Trehalose Rescues Postmenopausal Osteoporosis Induced by Ovariectomy through Alleviating Osteoblast Pyroptosis via Promoting Autophagy" Biomedicines 12, no. 10: 2224.
https://doi.org/10.3390/biomedicines12102224
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