Oroxylum indicum Seeds—Analysis of Flavonoids by Micellar Electrokinetic Chromatography
Abstract
:1. Introduction
2. Experimental Section
2.1. Chemicals, References and Plant Material
2.2. Method Validation
2.3. Sample Preparation
2.4. CE-Method
3. Results and Discussion
3.1. Method Development
3.2. Method Validation
Regression equation | R2 | Range * | |
---|---|---|---|
1 | y = 0.12384x | 0.9991 | 2.56–621.96 |
2 | y = 0.07970x | 0.9997 | 2.40–673.49 |
3 | y = 0.21337x | 0.9998 | 3.69–896.34 |
4 | y = 0.11283x | 0.9995 | 8.69–704.20 |
5 | y = 0.42260x | 0.9994 | 1.33–973.08 |
6 | y = 0.60629x | 0.9994 | 1.37–999.52 |
7 | y = 0.32222x | 0.9998 | 6.99–782.89 |
LOD * | LOQ * | Precision (intra-day) | Precision (inter-day) | Accuracy (high) | Accuracy (medium) | Accuracy (low) | |
---|---|---|---|---|---|---|---|
1 | 3.20 | 9.69 | 0.35% | 6.90% | - | - | - |
2 | 5.31 | 15.03 | 1.44% | 2.72% | - | - | - |
3 | 1.86 | 5.62 | 0.70% | 4.09% | - | - | - |
4 | 3.51 | 10.64 | 0.53% | 5.78% | - | - | - |
5 | 0.94 | 2.84 | 3.32% | 6.11% | 99.1% | 99.1% | 97.6% |
6 | 0.52 | 2.35 | 1.86% | 5.83% | 100.9% | 97.5% | 99.3% |
7 | 1.24 | 4.82 | 1.54% | 6.64% | - | - | - |
3.3. Sample Analysis
4. Conclusions
Conflict of Interest
References
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Krüger, A.; Ganzera, M. Oroxylum indicum Seeds—Analysis of Flavonoids by Micellar Electrokinetic Chromatography. Chromatography 2014, 1, 1-8. https://doi.org/10.3390/chromatography1010001
Krüger A, Ganzera M. Oroxylum indicum Seeds—Analysis of Flavonoids by Micellar Electrokinetic Chromatography. Chromatography. 2014; 1(1):1-8. https://doi.org/10.3390/chromatography1010001
Chicago/Turabian StyleKrüger, Anja, and Markus Ganzera. 2014. "Oroxylum indicum Seeds—Analysis of Flavonoids by Micellar Electrokinetic Chromatography" Chromatography 1, no. 1: 1-8. https://doi.org/10.3390/chromatography1010001