Effect of Fermented Soy Beverage on Equol Production by Fecal Microbiota
Abstract
:1. Introduction
2. Materials and Methods
2.1. Collection of Fecal Samples
2.2. Identification of EP Fecal Microbiota
2.3. Identification of the Tetrahydrodaidzein Reductase (tdr) Gene in Fecal Samples
2.4. Kinetics of Equol Production by the Fecal Microbiota
2.5. Study of Equol Degradation by Fecal Microbiota
2.6. B. pseudocatenulatum and S. isoflavoniconvertens Culture Conditions
2.7. Soy Beverages Preparation and Isoflavone Analysis
2.8. Effect of Soy Beverages on Equol Production by S. isoflavoniconvertens DSM 22006 in a Colonic Model
2.9. Effect of Soy Beverages on Equol Production by Fecal Microbiota in a Colonic Model
2.10. Statistical Analysis
3. Results and Discussion
3.1. Selection of EP Fecal Microbiota
3.2. Kinetics Production and Degradation of Equol by Fecal Microbiota
3.3. Effect of SB and FSB on Equol Production by S. isoflavoniconvertens
3.4. Effect of SB and FSB on the Production of Equol by EP Fecal Microbiotas
4. Conclusions
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Compounds | Molecular Formula | [M − H]—(m/z) |
---|---|---|
3,4-dihydroxyphenylacetic acid | C8H8O4 | 1,670,350 |
4-Hydroxyphenylacetic acid | C8H8O3 | 1,510,401 |
3-(3,4-Dihydroxyphenyl) propionic acid | C9H10O4 | 1,810,506 |
2-(4-hydroxyphenyl)-propionic acid | C9H10O3 | 1,650,557 |
Protocatechuic acid | C7H6O4 | 1,530,193 |
Catechol | C6H6O2 | 1,090,295 |
3-(3-Hydroxy-4-methoxyphenyl) propionic acid | C10H12O4 | 1,950,663 |
Fecal Samples (FS) | Equol (>1 µM) | O-DMA (> 1 µM) | tdr | Individual Characteristics |
---|---|---|---|---|
FS1 | − | + | + | Female, 30–50 years |
FS2 | − | + | + | Female, 30–50 years |
FS3 | − | − | + | Female, 30–50 years |
FS4 | + | + | + | Female, >50 years |
FS5 | + | + | + | Female, <30 years |
FS6 | − | + | + | Female, <30 years |
FS7 | + | + | + | Male, 30–50 years |
FS8 | + | + | + | Female, <30 years |
FS9 | − | − | + | Male, >50 years |
FS10 | − | + | + | Female, >50 years |
FS11 | − | + | − | Female, 30–50 years |
FS12 | + | + | + | Male, 30–50 years |
FS13 | + | + | + | Male, >50 years |
FS14 | − | + | + | Male, >50 years |
FS15 | − | + | − | Male, <30 years |
FS16 | − | + | + | Female, 30–50 years |
FS17 | − | + | + | Female, >50 years |
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Ruiz de la Bastida, A.; Langa, S.; Curiel, J.A.; Peirotén, Á.; Landete, J.M. Effect of Fermented Soy Beverage on Equol Production by Fecal Microbiota. Foods 2024, 13, 2758. https://doi.org/10.3390/foods13172758
Ruiz de la Bastida A, Langa S, Curiel JA, Peirotén Á, Landete JM. Effect of Fermented Soy Beverage on Equol Production by Fecal Microbiota. Foods. 2024; 13(17):2758. https://doi.org/10.3390/foods13172758
Chicago/Turabian StyleRuiz de la Bastida, Ana, Susana Langa, José Antonio Curiel, Ángela Peirotén, and José María Landete. 2024. "Effect of Fermented Soy Beverage on Equol Production by Fecal Microbiota" Foods 13, no. 17: 2758. https://doi.org/10.3390/foods13172758