Phylogenetic Position of Shiraia-Like Endophytes on Bamboos and the Diverse Biosynthesis of Hypocrellin and Hypocrellin Derivatives
Abstract
:1. Introduction
2. Materials and Methods
2.1. Isolates
2.2. Morphological Analysis
2.3. DNA Extraction, PCR Amplification and Sequencing
2.4. Phylogenetic Analysis
2.5. Submerged Cultivation and Secondary Metabolite Extraction
2.6. HPLC-DAD-MS Analysis
3. Results
3.1. Phylogeny
3.2. Taxonomy
3.3. Hypocrellin Identification and Production
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Species | Specimen Voucher | Location | GenBank Accession Numbers | ||||
---|---|---|---|---|---|---|---|
ITS | LSU | SSU | TEF | RPB2 | |||
Didymocyrtis banksiae | CBS 142523 * | Australia | KY979757 | KY979812 | — | — | KY979850 |
Neoshiraia camelliae | NTUCC 18-092-1 * | China, Taiwan | MT112286 | MT071262 | MT071213 | MT743267 | MT513982 |
NTUCC 18-092-2 | China, Taiwan | MT112287 | MT071263 | MT071214 | MT743268 | MT513981 | |
Neoshiraia taiwanensis | NTUCC 17-031 * | China, Taiwan | MT112285 | MT071261 | MT071212 | MT114404 | MT743276 |
NTUCC 18-091-5 | China, Taiwan | MT112280 | MT150600 | MT071207 | MT114405 | MT434762 | |
NTUCC 18-091-6 | China, Taiwan | MT112309 | MT150602 | MT071236 | MT425440 | MT434766 | |
NTUCC 18-091-7 | China, Taiwan | MT112310 | MT150601 | MT071237 | MT425441 | MT434765 | |
Phaeosphaeria chiangraina | MFLUCC 13-0231 * | Thailand | KM434270 | KM434280 | KM434289 | KM434298 | KM434307 |
Phaeosphaeria oryzae | CBS 110110 * | South Korea | KF251186 | KF251689 | GQ387530 | — | KF252193 |
Phaeosphaeria sp. | 20081120 | China, Yunnan | HQ324780 | — | — | — | — |
Pleospora herbarum | CBS 191.86 * | India | KC584239 | DQ247804 | GU238232 | DQ471090 | DQ247794 |
Pleosporales sp. | MX286 | — | JQ905814 | — | — | — | — |
Phaeosphaeriopsis agavacearum | CPC 29122 * | Australia | KY173430 | KY173520 | — | — | KY173591 |
Poaceicola italica | MFLUCC 13-0267 | Italy | KX926421 | KX910094 | KX950409 | MG520924 | KX891169 |
Populocrescentia forlicesesensis | MFLU 15-0651 * | — | KT306948 | KT306952 | KT306955 | MG520925 | — |
Pseudoshiraia conidialis | zzz816 | China, Guangxi | HQ696072 | MZ519516 | HQ696106 | MZ516164 | MZ516177 |
zzz510 | China, Guangxi | HQ696078 | MZ519517 | MZ519540 | MZ516165 | — | |
CNUCC 0316 | China, Guangxi | MZ519529 | MZ519521 | MZ519536 | — | — | |
CNUCC 0334 | China, Guangxi | MZ519528 | MZ519520 | MZ519535 | MZ516160 | — | |
CNUCC 0019 | China, Guangxi | MZ519532 | MZ519523 | MZ519538 | MZ516162 | — | |
CNUCC 0164 | China, Guangxi | MZ519530 | MZ519522 | MZ519537 | MZ516161 | MZ516175 | |
CNUCC 1353PR * | China, Yunnan | MZ519527 | MZ519534 | MZ519533 | MZ516168 | — | |
CNUCC 0006 | China, Anhui | MZ519531 | MZ519524 | MZ519539 | MZ516163 | MZ516176 | |
JAP103846 | Japan | MZ519526 | MZ519519 | MZ519542 | MZ516167 | MZ516179 | |
JAP103847 | Japan | MZ519525 | MZ519518 | MZ519541 | MZ516166 | MZ516178 | |
JP93 | Japan | AB255277 | AB354975 | — | — | — | |
JP256 | Japan | AB354995 | AB354980 | — | — | — | |
JP232 | Japan | AB255303 | AB354979 | — | — | — | |
JP7 | Japan | AB255241 | AB354974 | — | — | — | |
SUPER H168 | China | EU267793 | — | EU267792 | — | — | |
Rubroshiraia bambusae | HKAS102255 * | China | MK804678 | MK804658 | MK804704 | MK819218 | — |
HKAS102256 | China | MK804679 | MK804659 | MK804705 | MK819219 | — | |
HKAS102260 | China | MK804680 | MK804660 | MK804706 | MK819220 | — | |
HKAS102268 | China | MK804681 | MK804661 | MK804707 | MK819221 | — | |
HKAS102269 | China | MK804682 | MK804662 | MK804708 | MK819222 | — | |
HKAS102270 | China | MK804683 | MK804663 | MK804709 | MK819223 | — | |
HKAS102271 | China | MK804684 | MK804664 | MK804710 | MK819224 | — | |
HKAS102272 | China | MK804685 | MK804665 | MK804711 | MK819225 | — | |
HKAS102273 | China | MK804686 | MK804666 | MK804712 | MK819226 | — | |
HKAS102274 | China | MK804687 | MK804667 | MK804713 | MK819227 | — | |
BJTC HOU1000 | China, Yunnan | MZ497359 | MZ497374 | MZ497385 | MZ516169 | — | |
CNUCC 1000 | China, Yunnan | MZ497360 | MZ497376 | MZ497387 | MZ516170 | MZ516180 | |
Sclerostagonospora ericae | CPC 25927 * | South Africa | KX228268 | KX228319 | — | — | — |
Shiraia bambusicola | HKAS102253 | China | MK804668 | MK804648 | MK804694 | MK819208 | MK819228 |
HKAS102254 | China | MK804669 | MK804649 | MK804695 | MK819209 | MK819229 | |
HKAS102257 | China | MK804670 | MK804650 | MK804696 | MK819210 | MK819230 | |
HKAS102261 | China | MK804671 | MK804651 | MK804697 | MK819211 | MK819231 | |
HKAS102262 | China | MK804672 | MK804652 | MK804698 | MK819212 | MK819232 | |
HKAS102263 | China | MK804673 | MK804653 | MK804699 | MK819213 | MK819233 | |
HKAS102264 | China | MK804674 | MK804654 | MK804700 | MK819214 | MK819234 | |
HKAS102265 | China | MK804675 | MK804655 | MK804701 | MK819215 | MK819235 | |
HKAS102266 | China | MK804676 | MK804656 | MK804702 | MK819216 | MK819236 | |
HKAS102267 | China | MK804677 | MK804657 | MK804703 | MK819217 | MK819237 | |
NBRC 30312 | Japan | AB354982 | AB354963 | ||||
NBRC 30147 | Japan | AB354981 | AB354962 | ||||
NBRC 30737 | Japan | AB354983 | AB354964 | ||||
GZAAS2.0709 | China | GQ845413 | KC460983 | — | — | — | |
GZAAS2.0629 | China | GQ845415 | KC460980 | — | — | — | |
GZAAS2.0703 | China | GQ845412 | KC460981 | — | — | — | |
GZAAS2.0708 * | China | GQ845414 | KC460982 | — | — | — | |
BJTC HOU999 | China, Zhejiang | MZ497363 | MZ497378 | MZ497389 | MZ516171 | — | |
CNUCC 122 | China, Zhejiang | MZ497367 | MZ497382 | MZ497391 | MZ516174 | — | |
CNUCC 172 | China, Zhejiang | MZ497366 | MZ497381 | MZ497390 | MZ516173 | — | |
CNUCC MJ1 | China, Zhejiang | MZ497365 | MZ497380 | MZ497392 | MZ516172 | — | |
Shiraia sp. | JP151 | Japan | AB255289 | AB354977 | — | — | — |
JP119 | Japan | AB354993 | AB354976 | — | — | — | |
JP185 | Japan | AB354994 | AB354978 | — | — | — | |
MSX60519 | — | MN970609 | — | — | — | — | |
slf14 | — | GQ355934 | — | HM049630 | — | — |
Primer Pairs | Initial Step (T, t) | Denaturation (T, t) | Annealing (T, t) | Elongation (T, t) | Cycles | Final Step (T, t) |
---|---|---|---|---|---|---|
ITSIF/ITS4 | 94 °C, 5 min | 94 °C, 30 s | 55 °C, 30 s | 72 °C, 45 s | 35 | 72 °C, 10 min |
LR0R/LR5 | 55 °C, 30 s | 72 °C, 90 s | ||||
NS1/NS4 | 52 °C, 30 s | 72 °C, 90 s | ||||
EF1-983F/EF1-2218R | 55 °C, 30 s | 72 °C, 60 s | ||||
fRPB2-5f/fRPB2-7cr | 50 °C, 30 s | 72 °C, 80 s |
Samples | HA | HB | SA | EA | EB | EC | Total Content |
---|---|---|---|---|---|---|---|
S. bambusicola BJTC HOU999 | 3.60 | 1.80 | 4.99 | 0.00 | 0.00 | 0.00 | 10.39 |
R. bambusae BJTC HOU1000 | 49.54 | 6.02 | 10.34 | 0.00 | 0.00 | 0.00 | 65.89 |
Samples | HA | HB | SA | EA | EB | EC | Total Content |
---|---|---|---|---|---|---|---|
zzz816 | 84.86 | 1.71 | 41.11 | 226.48 | 7.19 | 0.86 | 362.22 |
CNUCC 1353PR | 677.11 | 155.36 | 152.31 | 326.59 | 60.41 | 38.36 | 1410.13 |
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Tong, X.; Wang, Q.-T.; Shen, X.-Y.; Hou, C.-L.; Cannon, P.F. Phylogenetic Position of Shiraia-Like Endophytes on Bamboos and the Diverse Biosynthesis of Hypocrellin and Hypocrellin Derivatives. J. Fungi 2021, 7, 563. https://doi.org/10.3390/jof7070563
Tong X, Wang Q-T, Shen X-Y, Hou C-L, Cannon PF. Phylogenetic Position of Shiraia-Like Endophytes on Bamboos and the Diverse Biosynthesis of Hypocrellin and Hypocrellin Derivatives. Journal of Fungi. 2021; 7(7):563. https://doi.org/10.3390/jof7070563
Chicago/Turabian StyleTong, Xin, Qiu-Tong Wang, Xiao-Ye Shen, Cheng-Lin Hou, and Paul F. Cannon. 2021. "Phylogenetic Position of Shiraia-Like Endophytes on Bamboos and the Diverse Biosynthesis of Hypocrellin and Hypocrellin Derivatives" Journal of Fungi 7, no. 7: 563. https://doi.org/10.3390/jof7070563