GlPP2C1 Silencing Increases the Content of Ganodermalingzhi Polysaccharide (GL-PS) and Enhances Slt2 Phosphorylation
Abstract
:1. Introduction
2. Materials and Methods
2.1. Strains and Culture Conditions
2.2. Transformant Construction
2.3. Gene Expression Analysis
2.4. Western Blotting
2.5. Growth Rate Experiments under Different Stresses
2.6. Quantification of β-1,3-D-glucan
2.7. Determination of EPS and IPS
2.8. Quantification of Hyphal Branches
2.9. Statistical Analysis
3. Results
3.1. Cloning and Sequence Analysis of the GlPP2C1 Gene
3.2. Construction of GlPP2C1 RNAi Strains in G. lingzhi
3.3. GlPP2C1 Contributed to GL-PS Biosynthesis in G. lingzhi
3.4. GlPP2C1 Gene Silencing Increases Cell Wall Stress Tolerance in G. lingzhi
3.5. GlPP2C1 Gene Silencing Increases Slt2 Phosphorylation
3.6. GlPP2C1 Silencing Positively Regulates Slt2-Mediated GL-PS Biosynthesis in G. lingzhi
3.7. PP2C Was Involved in the Cell Wall Integrity Pathway Mediated by Slt2 in G. lingzhi
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Wang, Z.; Chen, J.-H.; Wang, L.-S.; Ding, J.; Zhao, M.-W.; Liu, R. GlPP2C1 Silencing Increases the Content of Ganodermalingzhi Polysaccharide (GL-PS) and Enhances Slt2 Phosphorylation. J. Fungi 2022, 8, 949. https://doi.org/10.3390/jof8090949
Wang Z, Chen J-H, Wang L-S, Ding J, Zhao M-W, Liu R. GlPP2C1 Silencing Increases the Content of Ganodermalingzhi Polysaccharide (GL-PS) and Enhances Slt2 Phosphorylation. Journal of Fungi. 2022; 8(9):949. https://doi.org/10.3390/jof8090949
Chicago/Turabian StyleWang, Zi, Ju-Hong Chen, Ling-Shuai Wang, Juan Ding, Ming-Wen Zhao, and Rui Liu. 2022. "GlPP2C1 Silencing Increases the Content of Ganodermalingzhi Polysaccharide (GL-PS) and Enhances Slt2 Phosphorylation" Journal of Fungi 8, no. 9: 949. https://doi.org/10.3390/jof8090949