Electroextraction of Insoluble Proteins from the Organic Matrix of the Nacreous Layer of the Japanese Pearl Oyster, Pinctada fucata
Abstract
:1. Introduction
2. Materials and Methods
2.1. Decalcification of the Nacreous Layer
2.2. Heat Extraction of Proteins from the NOS
2.3. Electroextraction of Proteins from the NOS
2.4. SDS-PAGE Analysis
3. Results
3.1. Decalcification of the Pearl Oyster Shell Nacreous Layer
3.2. EEP Protein Composition
3.3. Presence of Disulfide Bonds in EEPs
4. Discussion
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Funabara, D.; Miyashita, N.; Nagai, K.; Maeyama, K.; Kanoh, S. Electroextraction of Insoluble Proteins from the Organic Matrix of the Nacreous Layer of the Japanese Pearl Oyster, Pinctada fucata. Methods Protoc. 2019, 2, 37. https://doi.org/10.3390/mps2020037
Funabara D, Miyashita N, Nagai K, Maeyama K, Kanoh S. Electroextraction of Insoluble Proteins from the Organic Matrix of the Nacreous Layer of the Japanese Pearl Oyster, Pinctada fucata. Methods and Protocols. 2019; 2(2):37. https://doi.org/10.3390/mps2020037
Chicago/Turabian StyleFunabara, Daisuke, Natsumi Miyashita, Kiyohito Nagai, Kaoru Maeyama, and Satoshi Kanoh. 2019. "Electroextraction of Insoluble Proteins from the Organic Matrix of the Nacreous Layer of the Japanese Pearl Oyster, Pinctada fucata" Methods and Protocols 2, no. 2: 37. https://doi.org/10.3390/mps2020037