Synergistic Effect of Chitosan and Selenium Nanoparticles on Biodegradation and Antibacterial Properties of Collagenous Scaffolds Designed for Infected Burn Wounds
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Methods
2.2.1. Preparation of Both Collagen and Collagen–Chitosan Scaffolds
2.2.2. Preparation of Collagen and Collagen–Chitosan Scaffolds with SeNPs
2.2.3. Microstructure Imaging and Evaluation
2.2.4. Investigation of Collagen Secondary Structure
2.2.5. Swelling and Water Absorption Studies
2.2.6. Degradation in a Simulated Physiological Environment
2.2.7. Release Kinetics of SeNPs
2.2.8. Antibacterial Activity Evaluation
3. Results and Discussion
3.1. Microstructure Imaging and Evaluation
3.2. Investigation of Intact Collagen Secondary Structure
3.3. Swelling Kinetics
3.4. Degradation in a Simulated Physiological Environment
3.5. Release Kinetics of SeNPs
3.6. Antibacterial Testing
4. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Sample Name | Matrix composition | |||
---|---|---|---|---|
Collagen (%) | Chitosan (%) | SeCH (ppm) | SeCE (ppm) | |
C | 100 | - | - | - |
C/SeCH | 100 | - | 1, 5, 10 | - |
C/SeCE | 100 | - | - | 1, 5, 10 |
CCH | 50 | 50 | - | - |
CCH/SeCH | 50 | 50 | 1, 5, 10 | - |
CCH/SeCE | 50 | 50 | - | 1, 5, 10 |
Scaffold | Wavenumbers [cm−1] | Triple-Helix Ratio | |||||
---|---|---|---|---|---|---|---|
Amide A | Amide B | Amide I | Amide II | Amide III | Pyrrolidine | ||
C | 3312 | 2926 | 1651 | 1545 | 1236 | 1450 | 0.94 |
C/SeCH | 3325 | 2932 | 1657 | 1551 | 1236 | 1452 | 0.95 |
C/SeCE | 3319 | 2930 | 1657 | 1551 | 1238 | 1452 | 0.96 |
CCH | 3304 | 2925 | 1659 | 1553 | 1236 | 1451 | 0.70 |
CCH/SeCH | 3325 | 2928 | 1655 | 1551 | 1236 | 1454 | 0.87 |
CCH/SeCE | 3321 | 2935 | 1655 | 1553 | 1239 | 1452 | 0.80 |
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Dorazilová, J.; Muchová, J.; Šmerková, K.; Kočiová, S.; Diviš, P.; Kopel, P.; Veselý, R.; Pavliňáková, V.; Adam, V.; Vojtová, L. Synergistic Effect of Chitosan and Selenium Nanoparticles on Biodegradation and Antibacterial Properties of Collagenous Scaffolds Designed for Infected Burn Wounds. Nanomaterials 2020, 10, 1971. https://doi.org/10.3390/nano10101971
Dorazilová J, Muchová J, Šmerková K, Kočiová S, Diviš P, Kopel P, Veselý R, Pavliňáková V, Adam V, Vojtová L. Synergistic Effect of Chitosan and Selenium Nanoparticles on Biodegradation and Antibacterial Properties of Collagenous Scaffolds Designed for Infected Burn Wounds. Nanomaterials. 2020; 10(10):1971. https://doi.org/10.3390/nano10101971
Chicago/Turabian StyleDorazilová, Jana, Johana Muchová, Kristýna Šmerková, Silvia Kočiová, Pavel Diviš, Pavel Kopel, Radek Veselý, Veronika Pavliňáková, Vojtěch Adam, and Lucy Vojtová. 2020. "Synergistic Effect of Chitosan and Selenium Nanoparticles on Biodegradation and Antibacterial Properties of Collagenous Scaffolds Designed for Infected Burn Wounds" Nanomaterials 10, no. 10: 1971. https://doi.org/10.3390/nano10101971