Reprint

Biological Liquid-Liquid Phase Separation, Biomolecular Condensates, and Membraneless Organelles

Edited by
November 2023
202 pages
  • ISBN978-3-0365-8931-2 (Hardback)
  • ISBN978-3-0365-8930-5 (PDF)

This is a Reprint of the Special Issue Biological Liquid-Liquid Phase Separation, Biomolecular Condensates, and Membraneless Organelles that was published in

Biology & Life Sciences
Chemistry & Materials Science
Medicine & Pharmacology
Summary

This reprint presents recent developments in the field of biological liquid–liquid phase separation (LLPS, also known as biomolecular condensation). LLPS and related biogenesis of various membraneless organelles (MLOs) and biomolecular condensates (BMCs) represent fundamental molecular mechanisms governing the spatio-temporal organization of the intracellular space. In fact, MLOs and BMCs, being liquid droplets, represent specific compartments within a cell that are not enclosed by a lipid membrane. Most biological LLPS processes are reversible, and many MLOs/BMCs exist transiently; they rapidly emerge when conditions are changed and rapidly disintegrate as soon as the original conditions are restored, thereby showing a characteristic “now you see me, now you don’t” behavior. Numerous MLOs/BMCs are found inside eukaryotic cells, where they exist as liquid droplets (or cellular bodies, puncta, etc.) in the cytoplasm, nucleoplasm, mitochondrial matrix, and stroma of chloroplasts. Furthermore, MLOs/BMCs are commonly observed in Archaea, bacteria, and, likely, viruses. MLOs/BMCs have numerous crucial functions, and their biogenesis is known to be controlled by various external factors and environmental cues, such as changes in temperature, pH, and ionic strength of the solution. All of these have garnered the close attention of many researchers to biological LLPS, MLOs, and BMCs.

Format
  • Hardback
License and Copyright
© 2022 by the authors; CC BY-NC-ND license
Keywords
Alzheimer’s disease; amyloid aggregation; lipid bilayer; cholesterol; time-lapse AFM imaging; molecular dynamics; liquid–liquid phase separation (LLPS); membraneless organelles; phase-separated condensates; human diseases; liquid–liquid phase separation; membraneless organelles; intrinsically disordered proteins; proteins with low complexity; P-body; liquid–liquid phase separation; Nst1; polyampholyte domain; aggregation-prone domain; Saccharomyces cerevisiae; liquid–liquid phase separation; membrane-less organelle; nuclear speckle; nucleolus; phase separation; chromatin organization; nuclear condensate; intrinsically disordered region; transcription; DNA damage repair; super-enhancer; quantitative imaging; CTP synthase; cytoophidium; fluorescence recovery after photobleaching (FRAP); stimulated emission depletion (STED); CTP synthase; cytoophidium; Drosophila; epithelium; follicle cell; ingression; paramyxoviruses; Hendra virus; intrinsically disordered proteins; amyloid-like fibrils; Taylor Dispersion Analysis (TDA); negative staining Transmission Electron Microscopy (ns-TEM); Polyethylene glycol (PEG) precipitation assays; Congo Red; Small-Angle X-ray Scattering (SAXS); actin; actin polymerization; actin-binding proteins; liquid–liquid phase separation (LLPS); coacervate; membrane; signaling proteins; n/a

Related Books

August 2022

Modeling and Simulation of Lipid Membranes

Biology & Life Sciences
...
November 2020

New Trends in Lyotropic Liquid Crystals

Chemistry & Materials Science
September 2019

Biological Crystallization

Biology & Life Sciences