*2.5. Cell Culture*

Human glioblastoma U87 MG cells (ATCC) were used for all experiments because of their highly invasive nature. Cells were cultured at 37 ◦C in a 5% CO2 atmosphere in DMEM/F12 (Sigma-Aldrich, St. Louis, MO, USA) supplemented with 10% fetal bovine serum (Fisher Scientific, Hampton, NH, USA), 1% penicillin/streptomycin (Invitrogen, Carlsbad, CA, USA) and 1% MycoZap (Life Technologies, Carlsbad, CA, USA). Cells were fed 2–3 times a week and passaged at confluence prior to use.
