*2.7. Statistical Analysis*

Statistical analysis of values related with antibody immobilization, TNF-α capturing profiles and biological studies was performed using IBM SPSS software (version 21; SPSS Inc., Armonk, NY, USA). Firstly, a Shapiro-Wilk test was used to ascertain the assumption of data normality. For antibody immobilization, protein capture and biological assays, the results showed that the data was not following a normal distribution. P values lower than 0.01 were considered statistically significant in the analysis of the results. A Kruskal-Wallis test was also performed for the comparison of more than two independent groups of samples for one variable.
