2.4.2. Fluorescence Microscopy

A fluorescence microscope (Axio Imager Z1m, Zeiss, Gottingen, Germany) was used to analyze the spatial distribution of the TNF-α antibody at the surface of electrospun NFMs. The TNF-α antibody was linked by a secondary antibody with green fluorescence (Alexa Fluor™ 488). Photographs were recorded at magnifications of 50, 200 and 400 times.
