2.6.4. Total Protein Synthesis Quantification

Total protein was performed using Micro BCA™ Protein Assay Kit (ThermoFisher Scientific, Rockford, IL, USA) according to the manufacturer´s instructions. Triplicates of each condition collected at 1, 14, 21, and 28 days were evaluated. The samples were collected as described in Section 2.6.3. A standard curve was prepared ranging from 0 to 200 μg/mL. The absorbance of each sample was measured at 562 nm using a microplate reader (Synergy HT, BioTek, Winooski, VT, USA).
