*3.1. Blend Electrospinning*

Blend electrospinning is the conventional electrospinning technique, in which the drug/biomolecules are dispersed or dissolved in the polymer solution, resulting in the development of nanofibers with drugs/biomolecules dispersed throughout the fibers [23]. Although this method is simple in contrast with other electrospinning methods, the solvents used for the dispersion of bioactive molecules can lead to protein denaturation or loss of biological activity. In addition, the inherent charge of the biomolecules can often result in their migration on the jet surface and thereby result in their distribution on the surface of the nanofibers rather than the encapsulation of biomaterials within the fibers. The surface dispersion can be associated with the burst release of the biomolecules [24].
