2.5.2. Determination of the pKa Values of PHEA*-g-*bAPAE Copolymer by Potentiometric Titration

30 mg of PHEA*-g-*bAPAE were dissolved in 0.1 N degassed NaCl (30 mL), used as ionic strength stabilizer, and termostated at 25 ◦C under argon atmosphere. The solution was then titrated using 0.05 N HCl until pH 3 under inhert conditions. Backward titrations were performed using 0.05 N NaOH. For all titrations an AMEL 631 differential electrometer was used, which was calibrated against a set of multiple standard buffers (2.50 ± 0.01 ≤ pH ≤ 10.00 ± 0.01). The pKa values of the amine groups were extrapolated using the De Levie method of acid−base chemical equilibria for polyelectrolytes.
