*2.2. Cell Culture*

MCF-7 and MDA-MB-231 as human breast cancer cell lines and 4T1 as a mouse breast cancer cell line were cultured in 75-cm2 tissue culture flasks (Nunc, Orlando, FL, USA) using DMEM supplemented with 10% FBS, 1% penicillin and streptomycin, and 1% HEPES at 37 ◦C in a humidified 5% CO2-containing atmosphere.
