*2.6. Confocal Laser Scanning Microscopy*

Biofilms of *Acetobacter* spp. were formed on glass slides by complete submersion of slides in inoculated media (50 mL) and incubation for 48 h. Selected slides were treated with CSA-131 (100 μg/mL) and slides were further incubated at 37 ◦C for 24 h. Following incubation, glass slides were carefully removed from the solutions and rinsed three times with PBS. Using protocols of a BacLight Live/Dead Viability Kit (L13152, Molecular Probes, Inc), biofilms were stained and further imaged by a confocal laser scanning microscope (Olympus FluoView FV1000) at ×60 magnification [12].
