*2.3. Bacterial DNA Extraction*

Total DNA for PCR were extracted using the boiled lysate method [32]. This was done by taking 200 μL of an overnight culture, mixing with 800 μL of sterile distilled water and boiling for 10 minutes. The resultant solution was centrifuged at 13,000 rpm for five minutes and the supernatant was used as a DNA template. This was kept at −20 ◦C for subsequent use.
