*2.4. Sequence Analysis*

Amplicons obtained in the different PCRs were sequenced to confirm the presence of integrons and insertion sequences. Bidirectional DNA sequence analysis was performed by the Macrogen EZ-Seq purification service (Macrogen Europe, Amsterdam, The Netherlands). Searches for DNA and protein homologies were carried out using the National Center for Biotechnology Information (http://www.ncbi.nlm.nih.gov/) using the BLAST program and the alignment of DNA and amino acids sequences were performed using Clustal Omega (http://www.ebi.ac.uk/Tools/msa/clustalo/).
