*3.1. Study Samples*

A total of 24 children were identified according to the inclusion criterion from the selected village. Stool samples from 22 children and drinking water from their respective home were collected. Samples from two children (one not at home, one did not passed motion by the time of collection) could not be collected even after two follow-up visits. Table 1 shows the demographic details of the families of the children from whom isolates were obtained. All of the isolates identified as blue-violet colonies on HiCrome agar were confirmed by PCR as *E. coli* while other bacterial isolates identified on HiCrome were considered together as the non-*E. coli* group and processed for antibiotic susceptibility testing.

The number and source of the samples and the number and types of coliforms isolated and studied from each sample is shown in Table 2.

**Table 1.** Socio-demographic characteristics of the families of included children (*n* = 22) in a village in Central India.


Types of house: walls, roof, and floors are made of bamboo, mud, grass, reeds, thatch, plastic/polythene, loosely-packed stone, etc., in Kachcha houses, stones, bricks packed with lime or cement mortar or concrete, in pucca houses, while in Semi-Pucca houses walls and roof are of concrete or un-burnt bricks, but the floor is made of mud or non-concrete items.

**Table 2.** Samples and commensal coliforms isolated from human and animal stool and water samples collected from a village in Central India.

