*2.4. Molecular Identification and Characterization of the Recovered E. coli Isolates*

Molecular identification of the presumptive *E. coli* isolates targeting the *uidA* gene and the various genes of the *E. coli* pathotypes screened were determined by following the method of Titilawo et al. [15] as shown in Table 1. The PCR products were resolved in a 2% (*w*/*v*) agarose gel in 1 × TAE buffer (40 mM Tris–HCl, 20 mM Sodium Acetate, 1 mM EDTA, pH 8.5), stained with 0.5 mg mL ethidium bromide (EtBr), and visualized under the Alliance BioDoc-It System (UFH, Alice 5700, South Africa) [23–25].



Titilawo et al. [15].

 conditions.
