*3.1. Antimicrobial Susceptibility Testing*

Both isolates revealed only resistance to tetracycline and tigecycline but stayed susceptible to all other tested antibiotics. Isolate MurTR-KL001 revealed a minimal inhibition concentration to tigecycline of 4 μg/mL and MurTR-KL002 of 8 μg/mL (Table 1).

#### *3.2. Genetic Analyses*

The two isolates belonged to two different unrelated MLST types: ST2392 (rpoB:1, gapA:2, mdh:172, pgi:1, phoE:9, infB:1, tonB:116) and ST2394 (rpoB:4, gapA:126, mdh:1, pgi:1, phoE:4, infB:3, tonB:351). Both MLST profiles had not been described prior to our study. Notably, *tonB* of MurTR-KL002 revealed a new allele (*tonB* 351) (Table 1).

**Table 1.** Characterization of tigecycline-resistant *Klebsiella pneumoniae* isolates.

