*3.4. Protein Content*

Protein content was determined according to the Bradford method [32]. The protein solution was stained with Coomassie Brilliant Blue G-250 and the absorption peak was observed at 595 nm wavelength [33]. The absorption value of A595 was directly proportional to the protein concentration. The standard curve of bovine serum protein (BSA) was used as a standard protein. The concentration of sample protein was calculated according to the standard curve.
