Effect on Mycelial Growth

The antifungal activity of the compounds was evaluated in vitro as described by Caruso et al. [13]. Compounds were dissolved in acetone and then added to Petri dishes along with malt yeast agar medium. Inhibition percentages were calculated after 72 h of incubation. Antifungal activity was expressed as the concentration that reduced mycelial growth by 50% (EC50), calculated by regressing the antifungal activity percentage against compound concentration. These experiments were done at least in triplicate.
