*4.3. Preparation of cell-free extracts*

Cells were sedimented by centrifugation (3220× *g*, 15 min). The biomass was resuspended in 3 mL of 50 mM potassium phosphate buffer (pH 7.2). The cells were disrupted by pulse-mode sonication (3 min duration and 1 s cycles) at 4 ◦C. Cell debris was removed by centrifugation (4 ◦C, 16,100× *g*, 15 min).
