*4.4. MIC/MBC Test*

The MIC test was assessed according to the process of the Clinical and Laboratory Standards Institute. The bacterial strains were diluted with MHB medium, and the concentration was <sup>5</sup> × 105 CFU/mL. Then, 180 <sup>μ</sup>L bacterial suspension was transferred to 96-well cell plates. The AMPs were diluted with phosphate-buffered solution (PBS), then 20 μL AMPs was added to the bacterial suspension, and the final concentrations were 200, 100, 50, 25, 12.5, 6.25, 3.125, and 1.56 μg/mL. Then, the bacteria were cultured at 37 ◦C for 8 h.
