*4.10. Ti Measurements*

Recombinant BglA enzymes in TBSx1 buffer (with and without the addition of 1 mM cellobiose) were used for Ti measurements, using a NanoTemper Tycho NT.6 instrument Agentek (1987) Ltd., Tel Aviv, Israel), according to the manufacturer's instructions [42].

#### **Supplementary Materials:** Supplementary material can be found at http://www.mdpi.com/1422-0067/20/19/4701/ s1.

**Author Contributions:** S.Y., Y.H., E.M. and E.A.B. conceived and designed the experiments; S.Y. performed the experiments and analyzed the data, M.A. and A.K. supported and advised the kinetic-parameter measurements, directed evolution and the screening method; O.S.-A. and F.F. resolved the 3D structure, S.Y., Y.H., J.S. and E.A.B. wrote the paper.

**Funding:** This research was supported by the United States–Israel Binational Science Foundation (BSF grant No. 2013284), Jerusalem, Israel; the Israel Science Foundation (ISF grant no. 1349/13); the European Union NMP.2013.1.1-2: CellulosomePlus Project number 604530, and by a research grant from the Yotam Project via the Sustainability and Energy Research Initiative (SAERI) at the Weizmann Institute of Science.

**Acknowledgments:** The authors are pleased to acknowledge the advice and assistance of Yoav Barak, Department of Chemical Research Support, The Weizmann Institute of Science. We also thank Itamar Kass (Ben-Gurion University of the Negev, Beer Sheva, Israel and Amai Proteins, Ltd., Rehovot, Israel) and Oren Yaniv (Beckman Coulter, Rehovot, Israel) for their insight into the structural aspects of BglA.

**Conflicts of Interest:** The authors declare no conflict of interest.
