**Low-Cost Cellulase-Hemicellulase Mixture Secreted by** *Trichoderma harzianum* **EM0925 with Complete Saccharification E**ffi**cacy of Lignocellulose**

**Yu Zhang 1, Jinshui Yang 1, Lijin Luo 2, Entao Wang 3, Ruonan Wang 1, Liang Liu 1, Jiawen Liu <sup>1</sup> and Hongli Yuan 1,\***


Received: 9 November 2019; Accepted: 2 January 2020; Published: 7 January 2020

**Abstract:** Fermentable sugars are important intermediate products in the conversion of lignocellulosic biomass to biofuels and other value-added bio-products. The main bottlenecks limiting the production of fermentable sugars from lignocellulosic biomass are the high cost and the low saccharification efficiency of degradation enzymes. Herein, we report the secretome of *Trichoderma harzianum* EM0925 under induction of lignocellulose. Numerously and quantitatively balanced cellulases and hemicellulases, especially high levels of glycosidases, could be secreted by *T. harzianum* EM0925. Compared with the commercial enzyme preparations, the *T. harzianum* EM0925 enzyme cocktail presented significantly higher lignocellulolytic enzyme activities and hydrolysis efficiency against lignocellulosic biomass. Moreover, 100% yields of glucose and xylose were obtained simultaneously from ultrafine grinding and alkali pretreated corn stover. These findings demonstrate a natural cellulases and hemicellulases mixture for complete conversion of biomass polysaccharide, suggesting *T. harzianum* EM0925 enzymes have great potential for industrial applications.

**Keywords:** glycoside hydrolyase; *Trichoderma harzianum*; complete saccharification; lignocellulose
