**5. Conclusions**

Under induction of lignocellulosic biomass, *T. harzianum* EM0925 could rapidly secrete glycoside hydrolases, including numerically and quantitatively balanced cellulases and hemicellulases and especially high levels of glycosidases. *T. harzianum* EM0925 enzyme cocktail presented significantly higher enzyme activities than the commercial preparations under the conditions tested, and released 100% of glucose and xylose from UGCS and ALKCS simultaneously. These findings clearly indicate that *T. harzianum* EM0925 enzyme cocktail has great potential for industrial applications.

**Supplementary Materials:** The following are available online at http://www.mdpi.com/1422-0067/21/2/371/s1, Table S1: A comparison of different previous reported enzyme cocktails for lignocellulose saccharification. Figure S1: Distribution of glycoside hydrolase family in proteome of *T. harzianum* EM0925.

**Author Contributions:** Conceptualization, Y.Z. and H.Y.; methodology, Y.Z. and L.L. (Lijin Luo); software, Y.Z. and L.L. (Liang Liu); validation, J.Y., E.W., and H.Y.; formal analysis, R.W.; investigation, J.L.; data curation, Y.Z., R.W., and H.Y.; writing—original draft preparation, Y.Z.; writing—review and editing, E.W. and H.Y.; visualization, J.Y.; supervision, H.Y.; project administration, J.Y. All authors have read and agreed to the published version of the manuscript.

**Funding:** This research was funded by the project for extramural scientists of state key laboratory of agrobiotechnology, grant number 2018SKLAB6-28.

**Conflicts of Interest:** The authors declare no conflict of interest.
