*4.8. Extraction of Amino Acid Signature from the Alignments and Visualization of Respective Positions on the three-dimensional (3D) Model of S. cerevisiae Isomaltase IMA1*

Protein sequences of *S. cerevisiae* maltase MAL62 (UniProtKB: P07265), *S. cerevisiae* isomaltase IMA1 (UniProtKB: YGR287C), *O. polymorpha* maltase-isomaltase MAL1 (UniProtKB: Q9P8G8) and *B. adeninivorans* AG2 were aligned using Clustal Omega [72] and amino acids corresponding to IMA1 signature positions determining the substrate specificity [12,15] were extracted from the alignment. The *S. cerevisiae* IMA1 structure in complex with isomaltose (PDB: 3AXH) [29] was visualized with PyMol [30] and amino acid signature was designated on the structure.
