*2.1. Materials*

Polyethylene glycol, poly (vinyl alcohol) 87%–89% hydrolyzed, coconut oil, ethylcellulose, hydrochloric acid, anhydrous sodium phosphate dibasic, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), Trypsin—EDTA, trypan blue, dimethylsulfoxide (DMSO), camptothecin, potassium dihydrogen phosphate monobasic bu ffer salt, isoniazid, and phosphate bu ffered saline powder were purchased from Sigma Chemical Company (St. Louis, MO, USA). D-fructose was obtained from Merck Chemicals (Darmstadt, Germany). The human breast cancer (MCF-7) cell line was purchased from American Type Culture Collection (ATCC, Manassas, VA, USA). Dulbecco's Modified Eagle's Medium (DMEM) and fetal bovine serum (FBS) were procured from BD Biosciences (San Jose, CA, USA). All other reagents utilized were of analytical grade and used as received from the supplier.

#### *2.2. Formulation of the Reconstitutable Dry Suspension Employing a Direct Dispersion Emulsification Method*

The RDS was prepared using a direct dispersion emulsification technique coupled with lyophilization and dry milling. This involved emulsification of the aqueous phase, which contained 2% <sup>w</sup>/w polyethylene glycol, 9% <sup>w</sup>/w poly (vinyl alcohol), 4% <sup>w</sup>/w D-fructose, 10% <sup>w</sup>/w isoniazid dispersed in water with the non-aqueous phase made up of 3% <sup>w</sup>/w ethylcellulose in coconut oil under constant mechanical blending (Silverson Machines, Inc., East Longmeadow, MA, USA) at 6000 rpm over 5–10 min until a monophasic emulsion was produced. With D-fructose as a lyoprotectant and sweetener, the mono-phased emulsion was exposed to liquid nitrogen for 15 min until completely frozen. Thereafter, the frozen sample was lyophilized (Benchtop Pro Freeze Dryer, VirTis, SP Scientific, Stone Ridge, NY, USA) at a temperature of −60 ± 2 ◦C and pressure of 124 ± 2 mTorr over 96 h to produce a solid lyophilisate that was then dry milled using a laboratory scale grinder (Kinematica GMBH, Eschbach, Germany). The RDS was stored away in airtight, opaque containers until further testing.
