*2.13. Stability*

Sterile nanoemulsion (MM3; 5 mL) was stored in an amber colored container for a period of three months in the refrigerator (2–8 ◦C). Stability of MM3 was routinely evaluated by visual inspection of the samples initially on a daily and later on a weekly basis for pH, phase separation, flocculation or precipitation [22]. Stability of nanoemulsion was also checked by centrifugation (Remi Centrifuge, Mumbai, India) by 12,000 rpm for 30 min and then the clarity, phase separation and concentration of drug were investigated. Droplet size of the nanoemulsion upon storage was also determined to assess the stability in terms of drastic changes in the mean droplet diameter due to droplet coalescence or aggregation.
