**Appendix B**

### *Drug Di*ff*usion to the Surrounding Skin Tissue*

In order to estimate the amount of drug that diffused to the nearby tissue, skin blocks were treated with ciprofloxacin loaded on PVP-foils or a solution of the drug in water. The wound tissue, as well as the surrounding tissue, were extracted and analyzed (Figure A3). The kinetics showed that the drug accumulated first in the wound tissue and diffused successively to the nearby tissue. After approximately 16 h, similar concentrations were reached in both areas of the ex vivo tissue block.

**Figure A3.** Penetration of ciprofloxacin into the wound and surrounding tissue after the topical application of (**a**) ciprofloxacin-loaded PVP foils (250 μg/wound) and (**b**) a solution of ciprofloxacin base in water (13 μg/wound).
