*2.9. Mouse Tumour Model*

Mouse xenograft tumours were generated by subcutaneous injection of 0.5 × 10<sup>6</sup> PC3 cells (+/ −CAV1) either alone or mixed with 0.5 × 10<sup>6</sup> WMPY-1 cells (+/ −TRIAP1) onto the hind limb of male NMRI nude mice (total volume 50 μL) as previously described [11,21]. Animals of each experimental group received a single subcutaneous injection. For radiation therapy mice were anesthetized (2% isoflurane) and tumours were exposed to a single dose of 10 Gy ± 5% in 5 mm tissue depth (~1.53 Gy/min, 300 kV, filter: 0.5 mm Cu, 10 mA, focus distance: 60 cm) using a collimated beam with an XStrahl RS 320 cabinet irradiator (XStrahl Limited, Camberly, Surrey, Great Britain). Mouse experiments were carried out in strict accordance with the recommendations of the Guide for the Care and Use of Laboratory Animals of the German Government and they were approved by the Committee on the Ethics of Animal Experiments of the responsible authorities [Landesamt für Natur, Umwelt und Verbraucherschutz (LANUV), Regierungspräsidium Düsseldorf Az.8.87-50.10.37.09.187; Az.8.87-51.04.20.09.390; Az.84-02.04.2015.A586].
