*2.4. qRT-PCR Analysis*

Total RNA was isolated from frozen cancer cell lines using Isogen (Nippon Gene, Tokyo, Japan), and 1 μg of total RNA was converted to cDNA with a first-strand cDNA synthesis kit (Amersham Biosciences Corp., Piscataway, NJ, USA). The qPCR was performed with a SYBR Select Master Mix (Applied Biosystems, Austin, TX, USA) as described previously [20]. ACTB-specific PCR products, which were amplified from the same RNA samples, served as internal controls. KIFC1 primer sequence: forward primer GACGCCCTGCTTCATCTG; reverse primer CCAGGTCCACAAGACTGAGG.
