*2.11. Immunohistochemistry and Immunofluorescence*

Immunohistochemistry was performed on 4 μm slides of formalin-fixed and paraffin-embedded prostate tissues after performing a descending alcohol-series and incubation for 10 min to 20 min in target retrieval solution (Dako, Agilent, Santa Clara, CA, USA) [11]. After blocking of the slides with 2% NGS/PBS sections were incubated with primary antibodies o/n at 4 ◦C. Antigen were detected with horseradish-peroxidase conjugated secondary antibodies (1:250) and developed with DAB (Dako). Nuclei were counterstained using haematoxylin.
