*2.8. Conditioned Media*

Cells were cultured in normal growth media until confluence. Cells were left non-irradiated or irradiated with 10 Gy, media were replaced and cells cultured in the presence of 0.5% foetal bovine serum for 48 h before collection of media. Control media were generated by incubating the same medium (containing 0.5% foetal bovine serum) without cells. Conditioned media were used as 1/1 mixture with normal growth medium [11,22].
