*2.5. Enzyme-Linked Immunosorbent Assay*

Serum PSA levels in mice were assayed using a PSA Enzyme Immunoassay test kit (Hope Laboratories, Belmont, CA, USA).

### *2.6. Preparation of Cell Lysates*

LNCaP, E9, F10, AIDL, and 22Rv1 cells were harvested by scraping, and whole cell lysates were prepared as previously described [14]. Briefly, the cell surface was washed with ice-cold phosphate-buffered saline and then lysed with CelLytic (Sigma-Aldrich Co., LLC.) containing 1% Nonidet P-40, 10 mM 4-(2-aminoethyl) benzensulfonyl fluoride, 0.8 mM aprotinin, 50 mM bestatin, 15 mM E-64, 20 mM leupeptin, and 10 mM pepstatin. After 60 min on ice, the lysates were centrifuged at 10,000× *g* for 10 min, and the supernatants were collected. The protein concentration was measured using a NanoDrop 2000 (Thermo Fisher Scientific Inc., Waltham, MA, USA).
