*2.7. Statistical Analysis*

Statistical analyses were performed using GraphPad Prism 7 (GraphPad Software, Inc., La Jolla CA, USA). All experiments were performed in triplicates for each biological sample. Data for cell proliferation assays, real-time RT-qPCR, and luciferase assays were depicted as mean ± SD from 3 to 4 independent experiments. IC50 values and 95% confidence intervals (CI) were calculated using the nonlinear regression analysis of percentage inhibition. The comparison of LogIC50 was calculated using the extra sum-of-squares F-test. One-way ANOVA followed by a Sidak's post hoc test was used to assess the difference between the data of real-time RT-qPCR and luciferase assay. *p* < 0.05 was considered to indicate a statistically significant difference.
