*2.5. Flow Cytometry Analysis*

To measure and quantify the DNA-fragmentation (apoptotic sub-G1 population), as well as to quantify the cell cycle phases, cells were incubated for 30 min at RT with a staining solution containing 0.1% (*w*/*v*) sodium citrate, 50 μg/mL PI and 0.05% (*v*/*v*) Triton X-100 (*v*/*v*). Afterwards they were analysed by flow cytometry (FACS Calibur, Becton Dickinson, Heidelberg, Germany; FL-2).
