*2.7. Western Blot Analyses*

Extracted proteins were separated by gel electrophoresis and transferred to Immobilon polyvinylidene difluoride membranes following our previously reported protocol [26]. Anti-AR, anti-PSA, anti-NSE, anti-phospho-STAT3, anti-STAT3, anti-phospho-Akt, anti-Akt, anti-phospho-p44/42 MAPK, anti-p44/42 MAPK, and anti-β-actin antibodies were used at dilutions of 1:2500, 1:5000, 1:5000, 1:2000, 1:2000, 1:1000, 1:1000, 1:2000, 1:1000, and 1:5000, respectively. Specific protein bands were assessed with a LAS-4000 Mini (Fuji Photo Film, Tokyo, Japan) using SuperSignal West Pico Chemiluminescent Substrate (Thermo Fisher Scientific Inc.).
