*5.14. Statistical Analysis*

Student's *t*, ANOVA, or Kruskal–Wallis tests were used to assess the association between variables. A value of *p* < 0.05 was considered statistically significant.

**Author Contributions:** All the authors have read and approved the manuscript and agree with its submission to this journal. Details regarding authorship, conflicts of interest, and ethics approval are given in the accompanying Author Submission Requirement Form. The contribution of each author to the manuscript sufficient enough for each to take public responsibility for appropriate portions of the content. T.E. made critical revisions of the manuscript. T.N., A.N.-I., T.S., H.K., Y.N., S.S. carried out the acquisition of data, and coordinated and helped to draft the manuscript. K.I. conducted statistical analyses concerning this study. T.Y., and S.T. supervised this manuscript. All authors read and approved the final manuscript.

**Funding:** This work was supported in part by a Grant-Aid from the Ministry of Education, Culture, Sports, Science, and Technology of Japan, gran<sup>t</sup> number 16K11023.

**Acknowledgments:** The authors would like to thank Hiroshi Takase for the assistance in the Core Laboratory of the Research Equipment Sharing Center at Nagoya City University.

**Conflicts of Interest:** The authors state that they have no potential conflict of interest to declare.
