*2.5. RNA Interference*

Silencer® Select (Ambion, Austin, TX, USA) against KIFC1 was used for RNA interference. Two independent oligonucleotides and negative control small interfering RNA (siRNA) (Invitrogen, Carlsbad, CA, USA) were used. Transfection was performed using Lipofectamine RNAiMAX (Invitrogen) according to the manufacturer's instructions. Cells were used 48 h after transfection in each of the experiments and assays [17].
