*2.12. Cell Viability*

The analysed peptide fractions and synthetic peptides were tested with MTT assay. Caco-2 cells (1 × 104) were seeded in 96 well plates and incubated with peptide fractions (0.125 mg/mL) or synthetic peptides (0.05 mg/mL). After 24 h, medium was removed and MTT solution (0.5 mg/mL, 100 μL) in PBS (1×) was added for 3 h in the dark at 37 ◦C. Removed MTT solution, the reaction was stopped with 100 μL of isopropanol/DMSO (9:1). The absorbance was followed (Abs595–690) using a plate reader (Tecan Infinite ® M200 PRO, Männedorf, Switzerland).
