*2.8. Wound-Healing Migration Assay*

To study the possibility that HLP alter migration of VSMC-treated TNFα, the cell medium was replaced with serum-containing medium following the treatments of TNFα (10 ng/mL) in the absence or presence various concentrations (0, 0.01, 0.05, and 0.10 mg/mL) of HLP, and the monolayers were wounded using scraping with a 20-μL pipette tip. At the indicated times (0, 24, and 48 h) after scraping, the above-treated cells were washed twice in PBS (pH 7.4). The cells were photographed using a phase-contrast microscope (Olympus, Tokyo, Japan) [23].
