*2.10. Cell Growth Curve Analysis*

VSMC was seeded into a 6-well culture plate at a density of 7 × 10<sup>4</sup> cells/mL, and then incubated with TNFα (10 ng/mL) in the absence or presence various concentrations (0, 0.2, and 0.5 mg/mL) of HLP for 24 h. The cell numbers were further counted using the Corning Cell Counter with a reusable glass counting chamber (Corning Inc, Corning, NY, USA) each day for 2 days. On the basis of the mean number of cells in these wells, the growth curves were formed.
