2.4.2. Oxygen Radical Absorbance Capacity

The oxygen radical absorbance capacity (ORAC) assay was performed according to Ganske and Dell [25]; the method was adapted to microplates and measurements were carried out with a microplate reader (POLARstar OPTIMA, BMG Labtech, O ffenburg, Germany). The samples were incubated for 10 min at 37 ◦C, and after incubation, 30 μL of 120 mM 2,2-azobis(2-amidinopropane) dihydrochloride (AAPH) solution was added rapidly using a POLARstar OPTIMA injector to trigger the oxidation reaction. The fluorescence was recorded every minute for 100 min. The filters used were 485 nm for λ excitation and 520 nm for λ emission. The ORAC values are expressed as μmol of Trolox Equivalents (TE) per gram of sample, using a standard calibration curve obtained by increasing concentrations of Trolox.
