2.15.1. Antioxidant Enzymes Detection

Superoxide dismutase (SOD1), thioredoxin reductase 1 (TrxR1), glutathione reductase (GR) and NAD(P)H quinone dehydrogenase 1 (NQO1) were evaluated by Western blot analysis, using 30 μg of cell protein lysates subjected to SDS-PAGE (12%). The WB detection was assessed using ECL system with UVITEC (Alliance Q9 Advanced, Cambridge, UK) equipment. Densitometric quantification of antioxidant enzymes WB was performed using NineAlliance software (Mini 9 17.01 version, Uvitec Alliance, Cambridge, UK). GAPDH was used as loading reference.
