*3.7. Microbiological Analyses*

Total coliforms, *Shigella* and *Salmonella* detection in the prototypes were performed in a BBLTM MacConkey II Agar (BD, Le Point Declaix, France), a selective and di fferential medium for the detection of coliforms and enteric pathogens. In addition, an XLD Agar (Xylose–Lysine–Deoxycholate Agar, BD, Le Point Declaix, France) was used to detect *Salmonella* and *Shigella*. Samples of each prototype were seeded on these agar plates and incubated at 37 ◦C for 24 h [69].

## *3.8. Statistical Analyses*

Sensory test data were analyzed with an analysis of variance (ANOVA) with α = 0.05. The PBP temperature stability data were gathered from two independent experiments, each in triplicate, and a non-parametric method was used in a stability test for comparisons of means values among groups (α = 0.05). The Shapiro–Wilk normality test was done to determinate if the dataset is well-modeled by a normal distribution. Since the data did not show a normal distribution, a Kruskal–Wallis test was performed.

This research was approved by the Research Ethics Committee, CEIC, Universidad de Antofagasta (Document 044/2017).
