**Phytochemistry and Comprehensive Chemical Profiling Study of Flavonoids and Phenolic Acids in the Aerial Parts of** *Allium Mongolicum* **Regel and Their Intestinal Motility Evaluation**

**Yongzhe Dong 1,2,**†**, Jingya Ruan 1,2,**†**, Zhijuan Ding 2, Wei Zhao 1, Mimi Hao 2, Ying Zhang 2, Hongyu Jiang 2, Yi Zhang 1,2,\* and Tao Wang 1,2,\***


Academic Editor: Ping-Chung Kuo Received: 15 December 2019; Accepted: 24 January 2020; Published: 29 January 2020

**Abstract:** To clarify whether flavonoids and phenols in *Allium mongolicum* Regel have the effect of improving gastrointestinal function and analyze its quality, this study was designed to isolate and identify them from the aerial parts of *A. mongolicum* by using various chromatographic and spectrophotometric methods, a bioassay on motility of mouse isolated intestine tissue, as well as qualitative analysis using liquid chromatography/mass spectrometry (LC-MS) analysis. As a result, 31 flavonoids and phenolic acids were obtained and identified, including six new flavonoid glycosides, mongoflavonosides A1 (**1**), A2 (**2**), A3 (**3**), A4 (**4**), B1 (**5**), B2 (**6**), and four new phenolic acid glycosides, mongophenosides A1 (**7**), A2 (**8**), A3 (**9**), B (**10**). Among them, eleven flavonoids and three phenolic acids showed significant increase in the height of mouse small intestinal muscle. It was a first systematic bioactive constituents' study for *A. mongolicum* on gastrointestinal tract. Furthermore, according to the retention time (*t*R) and the exact mass-to-charge ratio (*m*/*z*), thirty-one compounds were unambiguously identified by comparing to the standard references by using LC-MS. Then, on the basis of generalized rules of MS/MS fragmentation pattern, chromatographic behaviors, as well as biosynthetic laws of the 31 isolates, five flavonoid glycosides and one phenolic acid glycoside were tentatively speculated. On the basis of the study, a fast analysis method for flavonoids and phenolic acids in *A. mongolicum* was established.

**Keywords:** *Allium mongolicum* Regel; flavonoids; phenolic acids; mouse isolated intestine tissue; qualitative analysis
