*2.1. High-Performance Liquid Chromatography-Photodiode Array Detector-Electrospray Ionization*/*Mass Spectrometry Method Analysis of Ten Common Peeks*

Ten compounds (**1**)–(**10**) were found in fraction LM-P-1 to fraction LM-P-10 at the characteristic wavelength of 210 nm. All the constituents of 10 fractions were separated and detected within 80 min and their MS2 data were detected in a positive ion mode (Figure 2) according to the research of fragmentation pathway for compositions of 10 fractions in electrospray ionization using MS2 ion trap mass spectrometry and comparing retention time. Their structures were elucidated based on the analyses of ultraviolet (UV) spectra and ESI–MS2 fragmentation patterns with those of standards and the corresponding spectroscopic data given in the literatures.

**Figure 2.** The HPLC fingerprints of ten fractions.

An overview of identified compounds was shown in Table 1. Meanwhile, the detailed structural analysis of common peaks was taken as an example to illustrate that our paradigm in this part bore out the correctness of structural presumption by using MS2. The Fragmentation mode were consistent with the previously reported, it suggested that Compound (**2**) of m/z 368 [M+H]<sup>+</sup> was *N*-benzyl-(9z,12z,15z)-octadecatrienamide. The collision-induced dissociation (CID) spectra of (Figure 3) were displayed as examples for the illustration of fragmentation patterns of macamides.

**Figure 3.** The collision-induced dissociation (CID) spectra of a *N*-benzyl-(9z,12z,15z)-octadecatrienamide.

The five fragment ions (m/z 56, 96, 107, 136 and 260) which corresponded to butylene, (1Z, 4Z)-heptadecadiene, benzylamine, (1Z, 4Z, 7Z)-decatriene and (9Z, 12Z, 15Z)-octadecane-triene-ketone, respectively, through a classic α-cleavage in amide linkage, were detected in all standards and were considered as the diagnostic ions of macamides [2].


**1.**HPLC-ESI-MSdataof10common

#### *Molecules* **2019** , *24*, 2101
