Drug administration and blood collection

Male Sparague-Dawley rat (420 g) from National Laboratory Animal Center, Taipei, Taiwan were maintained under the regulations of the Institutional Animal Care and Use Committee of the National Health Research Institutes, Taiwan. The animal studies were supervised under the Institutional Animal Care and Use Committee, China Medical University, Taichung, Taiwan with a protocol number (CMUIACUC-2018-278). Rat was fasted for 16 h before dosing. Compound **2** was dissolved in aqueous solution (5% ethanol and 10% Tween80 in 85% saline) and administrated via oral gavage at a dosage of 100 mg/kg. Blood samples were collected at selected time points (5, 10, 20, 30, 60, 120, 240, 360, 480 min) post dose and centrifuged at 10,000× *g* for 15 min to provide serum samples, which were stored at −80 ◦C until subsequent sample extraction.
