*3.2. Materials and Methods for Bioassay*

The activities of compounds **1**–**31** were tested for frequency and height by using tissue perfusion method reported before [30]. Samples in DMSO solution were added after 15 min equilibrate incubation; the final DMSO concentration was 0.1% and final concentration of samples were 50 μM. Mosapride citrate dihydrate (Xi'an Janssen Pharmaceutical Ltd., Xi'an, China), final concentration was 200 μg/mL.

Data were analyzed by SPSS 22.0 software. All values were expressed as mean ± S.D. A *p*-value of 0.05 was considered to indicate statistical significance. One-way analysis of variance (ANOVA) and Tukey's studentized range test were used for the evaluation of the significant differences between means and post hoc, respectively.

#### *3.3. Materials and Methods for Qualitative Analysis*
