**1. Introduction**

Flavonoids and phenolic acids are secondary metabolites found in most *Allium* vegetables, such as onion (*Allium cepa* L.) [1], scallion (*Allium fistulosum* L.) [2], garlic (*Allium sativum* L.) [3], fruits, and traditional medicine. They also exert multiple biological properties, such as antitumor [4,5] antioxidant [6,7], anti-inflammation and gastrointestinal motility effect improvement [8–11], which make them show high correlations in the inhibition or management of many chronic diseases, such as cardiovascular and cerebrovascular diseases, diabetes, cancer, digestive system diseases, and so on.

As a traditional Mongolian medicinal herb, *Allium mongolicum* Regel (Liliaceae family) is mainly found in the high altitude desert of the Inner Mongolia Autonomous Region, Ningxia Hui Autonomous Region, Qinghai, Gansu, and Shanxi provinces [12], and has various special properties, such as stimulating the appetite, lowering blood pressure, hypolipidemic, replenishing the kidneys, and acting as an aphrodisiac [7]. Though it has been used to stimulate the appetite, studies were mainly focused on animal cultivation and improvement on meat products [12–14] up until now. Experiments related to the gastrointestinal tract have not been reported for the plant. On the other hand, pharmacological investigations showed the biological activities of *A. mongolicum*, including anti-inflammatory, antimicrobial, and antioxidant, which were attributed to the presence of different phytochemical groups like flavonoids and phenols [7,15,16]. However, it is still limited to the activity of total flavonoids and phenolic acids. Until now, the phytochemistry investigation of flavonoids is only reported by Dr. Zhao [16] and our laboratory [17,18]—there is no report for phenolic acids investigation. Moreover, there is no comprehensive chemical profiling study for the plant.

The present study was designed to isolate and identify flavonoids and phenolic acids from the aerial parts of *A. mongolicum* followed by bioactivity study on the motility of mouse isolated intestine tissue and qualitative analysis using liquid chromatography/mass spectrometry (LC-MS) analysis.
