*2.2. Inhibitory E*ff*ects of Obtained Compounds 1–31 on the Motility of Mouse Isolated Intestine Tissue*

Moreover, the obtained constituents of **1**–**31** were tested for frequency and height by using a tissue perfusion method [30]. Through tissue perfusion experiments, it was found that all compounds displayed no effect on isolated intestinal tissue contraction frequency (Table 3). While almost all isolates exhibited the tendency of increasing the contraction amplitude of mouse small intestinal muscle though only flavonoids **3**, **4**, **11**–**15**, **21**–**23**, and **26**, as well as phenolic acids **7**, **29**, and **30** showed significant difference comparing with normal group.


**Table 3.** Inhibitory effects of compounds **1**–**31** on motility of mouse isolated intestine tissue.

Values are the means ± standard error of measurement, significantly different from the control group, \* *p* < 0.05, \*\* *p* < 0.01, n = 6. Normal (N): isolated intestine tissue; Positive control (P): Mosapride citrate dihydrate, final concentration was 200 μg/mL. Compounds **1**–**31**: final concentration was 50 μM. Frequency, and height of normal group was set as 100%, relative values of them were calculated as: (sample/normal) × 100%.
