2.5.2. Morphology

Scanning electron microscopy (SEM) analysis of the electrospun fibers and the cryofractured annealed films was performed employing a Hitachi S-4800 microscope (Tokyo, Japan). The films were cryofractured using liquid nitrogen. The samples were attached to beveled holders using a double-sided adhesive tape, coated with a gold-palladium mixture under vacuum, and examined using an accelerating voltage of 5 kV. The average fiber diameter was determined using ImageJ program (National Institutes of Health, Bethesda, MD, USA).
