2.5.9. Antioxidant Activity

Dynamics of DPPH· free radical inhibition by the films was monitored. 1.9 mL of the DPPH· solution was added into a vial containing 1 mg of film sample. Control sample contained 1.9 mL of the DPPH· solution without film. Inhibition rate was determined by measuring the absorbance at 517 nm after incubation of the vials at 100 rpm in dark at room temperature for 0.5, 6, 12, and 24 h. The results were obtained following the Equation (1) and expressed as percentage of DPPH· inhibition. All measurements were performed in triplicate.
