*2.6. Statistical Analyses*

All graphical data are represented as the means ± SEM. Prior to analysis, data were determined to be normally distributed. All data were then analyzed using a 3-way analysis of variance ANOVA with genotype, epididymal segmen<sup>t</sup> and age as variables, followed by Tukey's multiple comparisons test. All statistical analyses were done with the software Prism8 v8.0.1 (GraphPad Software, La Jolla, CA, USA). Di fferences among samples were considered to be significant when the *p* < 0.05.
