*2.15. Statistical Analysis*

Statistical analysis was performed using Prism 8 statistical software. An ANOVA with a Tukey post-hoc test was used for the statistical evaluation of the results obtained from in vitro capacitation experiments. Multiple t-tests were performed for the computer-aided sperm analysis and acrosome exocytosis reaction experiments comparing different treatment groups under the same testing conditions. A t-test with Welch's correction was performed in the lipid peroxidation, total cellular ROS levels and in vitro fertilization experiments. All experiments were performed at least three times and all results are the averages of all replicates. For each experiment, all replicates of mouse experiments were performed with a different mouse, whereas all experiments performed in boar used a different ejaculate.
