*2.2. Sperm Extractions from Mice*

Spermatozoa were obtained from the fresh cauda epididymis' of mature CD1 and C57BL/6 males. Cauda epididymis' were placed in approximately 0.5 mL of phosphate-buffered saline (PBS) and pierced with a 26 12gauge needle to allow the sperm to diffuse out into the solution.
