**5. Conclusions**

In this study, a novel approach for fabricating a multilayered biodegradable bioengineered vascular construct with a cured structure and multi-branches was presented. The method used in this study combined 3D printed molds, casting hydrogel and sacrificial material, providing an easy technique to construct TEVGs whose morphology and structure are close to those of the original blood vessels. With the inherent advantages of 3D printing, it is envisioned that the proposed technique will play a significant role in the field of tissue engineering and in assisting studies on cardiovascular diseases.

**Supplementary Materials:** The following are available online at http://www.mdpi.com/2072-666X/10/4/275/s1, Video S1: Liquid perfusion inside the channels.

**Author Contributions:** Investigation, Y.L., Y.Z., W.J. and S.Z.; methodology, Y.L., Y.Z., H.P. and Y.P.; writing—original draft preparation, Z.Y., W.J.; writing—review and editing, Y.L., Y.P., J.L. and S.X.; validation, N.L., T.Y.

**Funding:** This research was funded by National Natural Science Foundation of China (grant number 51475281, 51575333) and China National Funds for Distinguished Young Scientists (grant number 61625304).

**Conflicts of Interest:** The authors declare no conflict of interest.
