*2.16. Immunofluorescence Analysis*

Brain sections were incubated with primary antibodies: anti-CD11b (1:100, abcam) or anti Iba-1 (1:100, Santa Cruz Biotechnology) in a humidified chamber at 37 ◦C overnight. Sections were washed with PBS and were incubated with secondary antibody FITC-conjugated anti-mouse Alexa Fluor-488 antibody (1:2000 *v*/*v* Molecular Probes, UK) for 1 h at 37 ◦C. Sections were laved and for nuclear staining <sup>4</sup>,6-diamidino-2-phenylindole (DAPI; Hoechst, Frankfurt; Germany) 2 μg/mL in PBS was added. Sections were analysed using a Leica DM2000 microscope [51].
