*2.5. Transmission Electron Microscopy*

The remnant kidney of uraemic animals was treated for ultrastructural analysis using a modified method of Rezzani et al. [28]. Briefly, renal tissue was fixed in 4% paraformaldehyde (pH 7.4) overnight at 4 ◦C and immersed in 2% osmium tetroxide at 4 ◦C for 1 hr. Tissue was dehydrated by immersion in graded ethanol and propylene oxide and embedded in Araldite-Epon resin. Representative blocks were taken subsequently with diamond knife and stained with uranyl acetate and lead citrate and observed using JEOL 2010 (JEOL, Inc, Peabody, MA, USA) high resolution transmission electron microscope at 80 kV to evaluate mitochondrial injury.
