*2.5. Western Blot*

Proteins were dissolved in RIPA bu ffer (Sigma-Aldrich, Darmstadt, Germany) with protease and phosphatase inhibitors (1:100, Sigma-Aldrich, Darmstadt, Germany). Samples were diluted in reducing Laemmli sample bu ffer and denatured (95 ◦C, 10 min). Following sample separation in a 12% SDS-PAGE, proteins were transferred onto an activated polyvinylidene difluoride membrane using a wet-blotting system. Membranes were blocked (1 h, 5% BSA/PBS-T) and incubated with the primary antibody (Supplementary Materials, Table S1, overnight, 5% BSA/PBS-T). Peroxidase-conjugated antispecies antibodies were used for detection (Supplementary Materials, Table S1, 1 h, PBS-T). WesternSure PREMIUM Chemiluminescent Substrate (LI-COR biosciences, Lincoln, NE, USA) visualized the antigen in a Fluor Chem FC2 Imaging System (Alpha Innotech, San Leandro, CA, USA).
