*2.13. Immunocytochemical Staining*

A549 (7 × 10<sup>4</sup> cells) grown on 24-well glass slides were exposed to LPS in the absence and presence of 10–100 μg/mL YE. A549 cells were fixed with 4% formaldehyde for 10 min and permeated with 0.1% Triton X-100 for 5 min on ice. Cells were blocked using a 4% FBS for 1 h. Immunofluorescent cytochemical staining of A549 cells was performed using NF-κB p50 antibody and Cy3-conjugated anti-rabbit IgG. Nuclear staining was performed with DAPI. Each slide was mounted in a VectaMount mounting medium and images were taken using an optical Axiomager microscope system.
