*2.11. Estimation of Catalase*

Twenty-one days after reserpine injection brain tissues were harvested and catalase activity was assayed by the method of Claiborne [48]. Briefly, the assay mixture consisted of 1.95 mL phosphate buffer (0.05 M, pH 7.0), 1.0 mL hydrogen peroxide (0.019 M), and 0.05 mL cytosolic fraction of brains in a final volume of 3.0 mL. Changes in absorbance were recorded at 240 nm. Catalase activity was assayed in terms of k min−1.
