4.3.2. 3D Cell Cultures

The experiments were conducted in culture media without antibiotics and with FCS. On day 7, cells in alginate beads were stimulated with 5 ng/mL IL-1β and collected after 24 h (day 1), eight days (day 8), and 15 days (day 15) (Table 5). Culture media for the latter group was exchanged on day 8, with new 5 ng/mL IL-1β. At the end of the experiment, the cells were liberated from the beads in 1.9 mL of 55 mM sodium citrate solution (71406, Sigma) and centrifuged at 700× *g* for 5 min. Cell pellets were used for subsequent analyses.


**Table 5.** Details of cell culture treatments.
