*4.5. Experimental Protocol*

A standard experiment included a 30 s application of conditioning solution, followed by 20 s of activating solution. Then, the process was repeated until at least three responses in a row were differing from each other by less than 10% of their amplitude. In experiments where the drug was applied during conditioning period, we had to reduce its potential effect on the open channels. To achieve this, channel activation was preceded bya3s flush of drug-free conditioning solution.

When the drug was applied only to the sustained current, both activating solution and the drug were applied until sustained current stabilized, with no other time constraints.

To account for response variability during the experiment, the control responses were averaged before drug application and after washout.
