*4.2. Streptozotocin-Induced Diabetes*

Animals were divided into equal groups: 20 mice treated with citrate buffer solution and 20 mice treated with streptozotocin (STZ, #S0130, Sigma-Aldrich, St. Louis, MO, USA). Diabetes was induced with a single intraperitoneal injection of STZ, at a fixed volume of 300 μL/animal, at the final concentration of 150 mg/kg/body weight in 0.05 mol/L sodium citrate buffer, pH 4.5, as previously described [37,55,56]. Citrate buffer solution was also injected intraperitoneal at a fixed volume of 300 μL/animal. Upon data analysis, the surviving animals from the STZ-injected group were divided into two subgroups: STZ-sensitive group and STZ-resistant group (see Results Section 2.1). The timeline of the experimental protocol is presented in Figure 7.

**Figure 7.** Timeline of the experimental protocol. Abbreviations W-weight, G-glycemia.

#### *4.3. Body Weight Measurements*

The body weight measurement was performed before the intraperitoneal injection (STZ or citrate buffer solution), and then repeated once per week (in the same day, at the beginning of the week, and at the same hour) for 8 weeks, as shown in Figure 2.

#### *4.4. Glycemia Measurements*

Blood glucose was measured from the tail vein blood by a glucometer (OneTouch, LifeScan, Milpitas, CA, USA). The blood glucose measurement was performed before the intraperitoneal injection (STZ or citrate buffer solution), and then repeated once per week (in the same day and at the same hour) for 7 weeks, as shown in Figure 1. Animals were fastened 12 h before the glycemia measurement. The body weight measurement was done alternately done before/after the glycemia measurement. In order to prevent any experimental bias, in the 8th week, the glycemia measurement was not performed, as animals were subjected to the hot-plate test.
