*4.4. Electrophysiology*

Electrophysiological experiments were performed 48–72 h after transfection. Green fluorescence detected with a Leica DMIL microscope was used to identify transfected cells. Current recordings were acquired with an EPC-8 (HEKA Elektronik, Lambrecht, Germany) patch clamp amplifier in whole-cell voltage-clamp mode at a membrane potential of −80 mV. The data were stored on a personal computer via Patchmaster software (HEKA Elektronik, Lambrecht, Germany). The recordings where access resistance and capacitance changed by more than 10% over the course of the experiment were excluded from the analysis.
