2.2.4. *PPT-A* mRNA Expression

In the ipsilateral TG and CSC, and in the medulla region, *PPT-A* mRNA expression levels were significantly increased in the IoN-CCI1 group compared with Sham1 and Sham2 groups (Figure 5). The increased mRNA levels were significantly reduced after treatment with ADM\_12 in IoN-CCI rats (IoN-CCI2 group) in the same regions (Figure 5). ADM\_12 administration did not cause any changes in sham-operated rats.

A significant difference was seen between the ipsi- and contralateral side (both in TG and CSC) in the IoN-CCI1 group, as well as in the IoN-CCI2 group at the TG level; whereas, there was no difference between groups on the contralateral side of TG and CSC (Figure 5A,B).

**Figure 5.** *PPT-A* mRNA expression in TGs (**A**), CSC (**B**), and medulla (**C**). Data is expressed as mean + SEM. One way analysis of variance (ANOVA) followed by Tukey's Multiple Comparison Test or Two-way ANOVA followed by Bonferroni post-hoc test, \* *p* < 0.05 and \*\*\* *p* < 0.001 vs. Sham1 and Sham2 groups (ipsi), ◦◦◦ *p* < 0.001 vs. IoN-CCI1 group (ipsi), ˆˆˆ *p* < 0.001 vs. IoN-CCI1 group (contra), §§§ *p* < 0.001 vs. IoN-CCI2 (contra).
