*2.2. Diabetic Mice Have Longer Paw Withdrawal Latencies to Nociceptive Thermal Stimulation than Diabetic Resistant or Control Mice*

We employed the hot-plate test at the fixed temperature of 55 ◦C and we measured the paw withdrawal latency in order to evaluate the thermal response in diabetic, diabetic resistant, and control mice groups (Figure 3). The two-way ANOVA analysis indicated statistical significance of the latency, of the diabetic condition, and of their interaction (Table S7). We compared the initial paw withdrawal latency (L0) measured at the beginning of the protocol (before any treatment), with the final paw withdrawal latency (Lf) measured after 8 weeks of the STZ-induction of diabetes. In the diabetic group, the final latency (Lf = 19.52 ± 3.52 s, *N* = 7) is significantly longer than the initial latency (L0 = 11.35 ± 1.69 s, unpaired *t*-test, *p* < 0.001). On the other hand an intergroup comparison of the final latency showed a statistical significant increase in the diabetic group (19.52 ± 3.52 s, *N* = 7) compared to the diabetic resistant group (11.36 ± 1.92 s, *N* = 6) or to the control group (11.03 ± 1.97 s, *N* = 20) (Table S8).
