*2.1. ADM\_12 Effect on Behavioral Response*

In agreement with Deseure and Hans [18], 5 days after surgery, the two groups of rats that underwent IoN-CCI displayed a lack of responsiveness to ipsilateral mechanical stimulation testing (MST) of the IoN territory (Figure 1A). At day 12, the hyporesponsiveness was recovering to be replaced at day +26 by a significant increase in the MST response score as compared to the two Sham groups (Figure 1A). On day +27, the administration of the TRPA1 antagonist treatment in operated rats (IoN-CCI2 group) reduced the response score of the mechanical stimulation compared to the IoN-CCI1 group (injected with saline) (Figure 1B); whereas, ADM\_12 treatment in sham-operated rats (Sham2 group) did not change the mechanical response. It is of note that the response to MST in the IoN-CCI2 group was significantly different between day +26 (before ADM\_12 injection) and +27 (after drug treatment) (Figure 1C).

**Figure 1.** Mechanical stimulation testing (MST): (**A**) Mean response score to Von Frey hair stimulation of the ligated/sham infraorbital nerve (IoN) territory, on pre-operative day (PO) and on +5, +12, +18, and +26 days post operation. Data is expressed as mean ± SEM. Two-way ANOVA followed by Bonferroni post-hoc test, \* *p* < 0.05 and \*\*\* *p* < 0.001 for chronic constriction injury of the infraorbital nerve (IoN-CCI) groups vs. Sham groups. Drug treatment effect on MST: (**B**) Mean response score to Von Frey hair stimulation on day +27, 1 h after ADM\_12 (or saline) treatment. Data is expressed as mean ± SEM. One-way ANOVA followed by Tukey's Multiple Comparison Test, \* *p* < 0.05 vs. Sham1 and Sham2, \*\*\* *p* < 0.001 vs. IoN-CCI1. (**C**) Comparison of the IoN-CCI2 group without treatment (day +26) and after ADM\_12 treatment (on day +27). Data is expressed as mean ± SEM. Paired Student's *t* test, §§§ *p* < 0.001 vs. day +26.
