2.2.3. *Calca* mRNA Expression

In the ipsilateral TG and CSC, and in the medulla region, *Calca* mRNA expression levels were significantly increased in the IoN-CCI1 group compared with Sham1 and Sham2 groups (Figure 4). Moreover, *Calca* mRNA levels in IoN-CCI1 and IoN-CCI2 groups were also significantly increased in the contralateral TG as compared to Sham groups (Figure 4A). The increased mRNA levels were significantly reduced after treatment with ADM\_12 in IoN-CCI2 rats in ipsilateral TG and CSC, and in medulla in toto (Figure 4). ADM\_12 administration did not provoke any changes in sham-operated rats (Figure 4).

**Figure 4.** *Calca* mRNA expression in TGs (**A**), CSC (**B**), and medulla (**C**). Data is expressed as mean + SEM. One way analysis of variance (ANOVA) followed by Tukey's Multiple Comparison Test or Two-way ANOVA followed by Bonferroni post-hoc test, \*\*\* *p* < 0.001 vs. Sham1 and Sham2 (ipsi), ◦◦ *p* < 0.01 and ◦◦◦ *p* < 0.001 vs. IoN-CCI1 (ipsi), ˆ *p* < 0.05 and ˆˆˆ *p* < 0.001 vs. IoN-CCI1 (contra), # *p* < 0.05 and ## *p* < 0.01 vs. Sham1 and Sham2 (contra).

A significant difference was seen between the ipsi- and contralateral side (both in TG and CSC) in the IoN-CCI1 group (Figure 4A,B).
