**4. Conclusions**

In summary, a database of 89 pesticides was built, including both chromatographic and HRMS information. The data was acquired after parameter optimization on ultrahigh performance liquid chromatography interfaced quadrupole-Orbitrap mass spectrometer. Based on the database, the screening rule for these compounds was further established by comparing their precursors, fragments, retention time and isotopes. The fast, high-throughput identification and rough quantification of these compounds was achieved. The method was successfully applied for the pesticides risk assessment of fishery drugs. However, as the detection mode established on potential and known pesticides, where their chromatographic and mass spectrometric information were examined and collected, the unknown, non-target risk compounds were ignored. Further work will be focused on non-target screening based on characteristic fragments for recognizing and monitoring risk factors. Overall, our current method can be used as a fast, reliable, e fficient and practical tools for the fishery drug risk assessment, which saves more time, and expenses.

**Supplementary Materials:** The following is available online, Table S1: The detailed linear profile for 82 compounds.

**Author Contributions:** C.K. and H.-J.Y. conceived and designed the experiments; S.-Y.W. and Q.-P.C. performed the experiments; C.K. and S.-Y.W. analyzed the data; C.K. and S.-Y.W. wrote the paper.

**Funding:** This research was funded by the National Natural Science Foundation of China (31701698), Shanghai Agriculture Applied Technology Development Program (Grant No. T2016010401), Shanghai Key Laboratory of Forensic Medicine (Academy of Forensic Science, KF1910).

**Acknowledgments:** C.K. thanks Wenlei Zhai for help in paper writing and revision.

**Conflicts of Interest:** The authors declare no conflict of interest.
