*4.14. HUVEC Proliferation Tests*

The cells were plated in six-well cell culture plates at 1 × 10<sup>5</sup> cells/well in 2 mL culture medium with CM. After 72 h of treatment at 37 ◦C, the cells were harvested by suspension in 0.025% trypsin with 0.02% EDTA. Cell counts were performed in triplicate while using a hemocytometer. Trypan blue exclusion assays were used to identify the viable cells. The cell number was determined and cell growth curves were generated.
