*4.3. Tissue Processing*

The animals were anesthetized with isoflurane, blood samples were collected via cardiac puncture for serum isolation, and the remaining livers were immediately removed. Part of the liver was fixed in 10% neutral-bu ffered formalin, embedded in para ffin, and sectioned for immunohistochemistry (IHC). The other liver tissue was used fresh for total protein extraction for BAFF determination by enzyme-linked immunosorbent assays (ELISAs) or for BCL10 determination by western blot analysis. Proteins were extracted while using Cell Lysis (Total Protein Extraction) bu ffer (Thermo Fisher Scientific, Waltham, MA, USA).
