*4.19. Western Blotting*

Protein concentrations in nuclear extracts from liver tissues or CL-48 cell lysates were quantified while using Bio-Rad protein assays (Hercules, CA, USA). Samples (10–50 μg protein) were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis, transferred onto polyvinylidene difluoride membranes, and immunoblotted with anti-BCL10 (sc-5273, dilution used 1:200; Santa Cruz Biotechnology) and anti-beta-Actin (sc-47778, dilution used 1:1000; Santa Cruz Biotechnology) antibodies. The entire western blots with marker were demonstrated in Supplementary Materials. The signals were detected by a Digital imaging system (Bio Pioneer Tech Co., New Taipei City, Taiwan). Meanwhile, the relative intensity of thesignals was analyzed with the ImageJ® program. Is the capitalization necessary? Additional examples will be highlighted below.
