*4.5. Macroscopic Analysis*

Evaluations were conducted with photographic and visual assessment in both groups. PU size (in mm2) was measured every seven days until complete healing (wound closure) using the software ImageJ (National Institute of Health, USA). The healing rate was calculated in both groups as the initial size of the ulcer in mm<sup>2</sup> per number of days until closure. The weight in grams of all mice was recorded before creating PU, after the three cycles of ischemia-reperfusion, and once per week until wound closure. All the measurements were taken by two independent researchers.
