*4.8. Statistical Analysis*

Wound groups were compared using two-factor repeated measures ANOVA. If three or more wound groups were compared, ANOVA was followed by post-hoc Tukey's test. Two wound groups were compared using Student's *t*-test or Welch's *t*-test after F test. *p* < 0.05 was considered significant. All statistical analyses were performed using Statcel2 software (OMS, Saitama, Japan).

**Author Contributions:** Conceptualization, M.A. and K.T.; methodology, M.A., H.Y. and K.T.; validation, M.A., H.A., P.M., T.T., N.M.M., E.T. and Y.O.; formal analysis, M.A.; investigation, M.A., H.A., P.M., T.T., N.M.M., E.T. and Y.O.; data curation, M.A.; writing—original draft preparation, M.A.; writing—review and editing, R.O. and K.T.; supervision, K.T.; project administration, K.T.; funding acquisition, M.A., T.T., and K.T.

**Funding:** This study was supported by R01 CA160688 from the National Institute of Health (NIH) and grants (Nos. JP16K20371 and JP17K17037) from the Japan Society for the Promotion of Science (JSPS).

**Conflicts of Interest:** The authors declare no conflict of interest.
