4.8.6. Image Acquisition

Images were obtained on a Zeiss fluorescence microscope (Nikon 4X NA 0.10 air, Nikon 10X NA 0.45 air) or with a VS120 virtual microscopy slide scanning system (Olympus, Tokyo, Japan, objectives Olympus 4X NA 0.16 air, Olympus 10X 0.4 air), either in brightfield mode (hematoxylin/eosin, IHC) or fluorescent mode (IF). From the scanned slides, representative images were acquired using the OlyVIA software 2.8 (Olympus). Exposure times, acquisition settings, and image brightness adjustments were done consistently for each respective staining including negative controls.
