*4.4. Tissue Density Measurements*

For each individual within a group (i.e., biological replicates per group, *n* = 4), three technical replicates of sca ffold samples were used. Sca ffolds were first separately photographed to measure tissue area, using ImageJ software version 1.51n (NIH, Bethesda, MD, USA; http://imagej.nih.gov/ij) and subsequently freeze dried and weighed to calculate tissue density (mg/mm3).
