*4.3. Cell Growth*

ADCSs were seeded onto 35 mm culture dishes at a density of 2 × 10<sup>5</sup> cells/dish. The medium was changed every 2 days, and they were cultured up to 7 days. Cells were collected every day and the numbers of cells were counted by a cell counter (CDA-500, Sysmex, Kobe, Japan). Saturation density was determined as the number of cells at confluence at day 7.
