*4.8. Imaging*

#### 4.8.1. Scanning Electron Microscopy (SEM)

Sca ffolds and native lung tissues were washed in Sorensen's phosphate bu ffer (0.1 M, pH 7.4) and fixed in 1.5% formaldehyde and 1.5% glutaraldehyde for 1 h. After washing with Sorensen's bu ffer, the samples were dehydrated with gradual increasing concentration of ethanol. Samples were critical point dried and sputtered with gold-palladium before being examined with electron microscopy Jeol JSM-7800F FEG-SEM at Lund University Bioimaging Center (LBIC).
