*4.15. Protein Array Analysis*

A Proteome Profiler Human Angiogenesis Antibody Array (cat. #ARY007; R&D Systems) was used for the detection of angiogenesis-related factors according to the manufacturer's instructions. Briefly, CM was first mixed with the detection antibody cocktail at room temperature for 1 h prior to being added to the array membrane. The membrane was then incubated overnight at 2–8 ◦C on a shaker. After washing, horseradish peroxidase-conjugated streptavidin was added to the membrane, followed by incubation for 30 min. at room temperature on a shaker. After washing, X-ray film and a chemiluminescence imaging system were used to detect and quantify the array signals.
