*2.4. Isolation and Identification of 6-epi-Monanchorin by 1H NMR Spectroscopy*

The strain CB1-14 was chosen for preparative isolation of target compounds. After incubation of 12 L medium at 28 ◦C for 7 days, the culture broth of *Vibrio* sp. strain CB1-14 was separated from cells by centrifugation. The 6-*epi*-monanchorin (**2**, Figure 1) was isolated from the EtOAc extracts of both cells and lyophilized culture broth using reverse-phase HPLC. The structure was exactly identified on the basis of 1H NMR and HRESIMS data by comparison with authentic sample [4]. As a result, it was found that the CB1-14 strain biosynthesizes 6-*epi*-monanchorin (**2**). Monanchorin itself was not found in this strain in amounts sufficient for NMR spectrum recording.
