3.4.2. MTT Assay

Cell suspension (1 × 103 cells/well) was incubated with different concentration of compounds during 24 h. After that, cell viability was determined by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide) method as manufacturer described (Sigma-Aldrich, St. Louis, MO, USA).
