*4.9. Live Cell Imaging*

For quantification of live cells in H2O2-induced oxidative stress, cells were stained with calcein (#R37601, Thermo Fisher Scientific, Carlsbad, CA, USA). Nuclei were stained with NucBlue Live Ready Probes Reagent (#R37605, Thermo Fisher Scientific, Carlsbad, CA, USA). Live cells were observed under the CX7 High-Content Screening (HCS) System (100×, Thermo Fisher Scientific, Carlsbad, CA, USA). Images were captured (25 images per well) and experiments were repeated four times. Green fluorescence indicated live cells.
