*4.5. Homology Modeling*

The generation of a theoretical model of the spatial structure of magnificamide, as well as the valuation of its physico-chemical characteristics, were performed using the specialized software MOE 2016.08 (Montreal, QC, Canada) [21]. Simulation of the molecular dynamics of magnificamide in an aqueous environment, as well as the physicochemical characteristics valuation of α-amylase inhibitors, were performed in Amber10: EHT force field.

#### *4.6. In Vitro Antimicrobial Activity Assay*

The antimicrobial activity of r-magnificamide was tested against Gram-positive (*Staphylococcus aureus* ATCC 21027 and *Bacillus subtilis* ATCC6633), Gram-negative bacteria (*Escherichia coli* VKPM B-7935 and *Pseudomonas aeruginosa* ATCC 27853), and the fungus *Candida albicans* KMM 455 by the agar dilution method. Microbial strains were taken from the American Type Culture Collection (ATCC), Russian National Collection of Industrial Microorganisms (VKPM), and Collection of Marine Microorganisms (KMM) (Pacific Institute of Bioorganic Chemistry FEB RAS). To obtain a microbial lawn, 0.1 mL of a cell suspension (0.5 <sup>×</sup> 108 cells/mL) was uniformly distributed on agar surface in Petri dishes (15 g/L tryptic soy broth, 2 g/L bacto yeast extract, 1 g/L glucose, and 20 g/L agar for bacteria; for fungi 10 g/L of glucose was added). Wells with a diameter of 6 mm were punched into the agar and filled with 100 μL of the peptide solution at concentrations 1, 5, 10, and 20 μM. The plates were then incubated for 18 h at 37 ◦C for bacteria, and at 30 ◦C for the fungus. Minimum inhibitory concentration was determined by measuring the clear zone of inhibition around each well. All assays were performed independently three times.
