3.5.3. Cell Viability Assay

Cell suspension (1 × <sup>10</sup><sup>3</sup> cells/well) was incubated with different concentration of compounds for 24 h. After that, cell viability was determined using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide) method, according to the manufacturer's instructions (Sigma-Aldrich, St. Louis, MO, USA). The results were presented as percent of control data, and concentration required for 50% inhibition of cell viability (IC50) was calculated.
