*2.2. Preliminary Identification of Potential Microorganism-Producers by Monitoring of the Compounds Giving Ion Peaks at m*/*z 212.17 by MALDI MS, Characteristic of 1 and 2 MS*

A preliminary screening procedure was carried out using Ultrafex III MALDI TOF/TOF mass spectrometer and Biotyper Software (Bruker Daltonics) to select isolates for further analyses. Sample preparation was carried out by "direct transfer" procedure (Ver. 2.0 Biotyper). Spectra were calibrated with external calibration by *Eschercihia coli* DH5 alpha standard and protein calibration standard I (Bruker Daltonics). The majority of identified bacterial strains were represented by *Vibrio* spp. and, thus, *Vibrio* was the dominant group of bacteria cultured from the mucous net of this polychaete. The occurrence of the compounds with a peak at *m*/*z* 212.17 in MALDI MS, presumably corresponding to 6-*epi*-monanchorin or monanchorin (**2** or **1**), are shown in the Table 1. In total there were eleven promising strains found, seven of which were identified as *Vibrio* spp. and gave this ion peak in MS. It should be noted that the data obtained by this method should be considered as preliminary and did not allow accurate identification, neither microorganisms nor target compounds.


**Table 1.** Taxonomic position of microorganism-producers and occurrence of compounds with *m*/*z* 212.17 ion peak by MALDI MS data.

nd, not detected; ad, ambiguous detected; +, detected.
