*3.4. Extraction and Isolation*

The main part of the isolation procedures of compounds from *Penicillium* sp. KMM 4672 was described in a previous paper [20]. The *n*-hexane–EtOAc (95:5, 74.0 mg) fraction was purified by LH-20 column (80 cm × 2 cm) with CHCI3 to yield **2** (12.5 mg) and **3** (14.3 mg). The *n*-hexane–EtOAc (40:60, 54.0 mg) fraction was purified by HPLC on a YMC ODS-AM column, eluting with MeOH–H2O (65:35), and then by HPLC on a YMC SIL column, eluting with MeOH–CHCl3 (5:95) to yield **1** (1.9 mg).

The main part of isolation procedures of compounds from *Aspergillus* sp. KMM 4676 were described in a previous paper [30]. The *n*-hexane–EtOAc (80:20, 59.4 mg) fraction was purified by HPLC on a YMC ODS-AM column, eluting with MeOH–H2O (65:35) to yield **5** (3.45 mg). Another *n*-hexane–EtOAc (80:20, 157.7 mg) fraction was purified by HPLC on a YMC ODS-AM column, eluting with MeOH–H2O (65:35), and then by HPLC on a YMC SIL column, eluting with MeOH–CHCl3–NH4Ac (10:90:1) to yield **4** (6.74 mg).

The fungal mycelia of *Aspergillus flocculosus* with the medium were extracted for 24 h with 15 L of EtOAc. Evaporation of the solvent, under reduced pressure, gave a dark brown oil (5.0 g), to which 250 mL H2O–EtOH (4:1) was added, and the mixture was thoroughly stirred to yield a suspension. It was extracted, successively, with hexane (150 mL × 2), EtOAc (150 mL × 2), and *n*-BuOH (150 mL × 2). After evaporation of the EtOAc layer, the residual materials (3.36 g) were passed over a silica gel column (35.0 cm × 2.5 cm), which was eluted with a hexane–EtOAc gradient (1:0–0:1). The *n*-hexane–EtOAc (75:25, 41.4 mg) fraction was purified by HPLC on a YMC SIL column, eluting with MeOH–CHCl3-NH4Ac (97:3:1), and then by HPLC on a YMC ODS-AM column, eluting with MeOH–H2O (75:25) to yield **6** (24.1 mg).

6-Hydroxy-*N*-acetyl-β-oxotryptamine (**1**): white powder; 1H and 13C NMR data see Table 1, Figures S1–S7; HR ESIMS *<sup>m</sup>*/*<sup>z</sup>* 231.0772 [M−H]– (calcd for C12H11N2O3, 231.0775, <sup>Δ</sup> + 1.4 ppm) (Figure S8).

#### *3.5. Biological Activity of Compounds*
