*Article* **New Trisulfated Steroids from the Vietnamese Marine Sponge** *Halichondria vansoesti* **and Their PSA Expression and Glucose Uptake Inhibitory Activities**

**Kseniya M. Tabakmakher 1, Tatyana N. Makarieva 1,\*, Vladimir A. Denisenko 1, Roman S. Popov 1, Pavel S. Dmitrenok 1, Sergey A. Dyshlovoy 1,2, Boris B. Grebnev 1, Carsten Bokemeyer 2, Gunhild von Amsberg 2,3 and Nguyen X. Cuong <sup>4</sup>**


Received: 26 June 2019; Accepted: 24 July 2019; Published: 27 July 2019

**Abstract:** Seven new unusual polysulfated steroids—topsentiasterol sulfate G (**1**), topsentiasterol sulfate I (**2**), topsentiasterol sulfate H (**3**), bromotopsentiasterol sulfate D (**4**), dichlorotopsentiasterol sulfate D (**8**), bromochlorotopsentiasterol sulfate D (**9**), and 4β-hydroxyhalistanol sulfate C (**10**), as well as three previously described—topsentiasterol sulfate D (**7**), chlorotopsentiasterol sulfate D (**5**) and iodotopsentiasterol sulfate D (**6**) have been isolated from the marine sponge *Halichondria vansoesti*. Structures of these compounds were determined by detailed analysis of 1D- and 2D-NMR and HRESIMS data, as well as chemical transformations. The effects of the compounds on human prostate cancer cells PC-3 and 22Rv1 were investigated.

**Keywords:** marine sponge; *Halichondria vansoesti*; trisulfated steroids; topsentiasterol sulfates; halistanol sulfates; anticancer activity; PSA expression; glucose uptake
