*2.1. Genome Sequencing and Assembly*

Among five currently described *Zobellia* species, there are four genomes available to the public on the NCBI database as of October 2019: *Z. galactanivorans* DsijT (PRJEB8976), *Z. galactanivorans* OII3 1c (PRJNA377409), *Z. uliginosa* DSM 2061<sup>T</sup> (PRJNA329763), and *Z. amurskyensis* MAR 2009 138 (PRJNA248513, revised from "*Z. uliginosa* MAR 2009 138"). However, only two genomes were obtained from type strains. We obtained two novel draft genomes of the other *Zobellia* species, deposited in the collection of marine microorganisms (KMM WDCM644) of PIBOC FEB RAS.

Type strains *Z. amurskyensis* KMM 3526<sup>Т</sup> and *Z. laminariae* KMM 3676<sup>Т</sup> were isolated from seawater (Amur Bay, Vladivostok, Russia) and brown alga *Laminaria japonica* (Troitsa Bay, Zarubino, Russia), respectively, and validly described by Nedashkovskaya et al. [33]. Draft genomes of both flavobacteria were obtained using Roche-454 technology; additionally, the genome of *Z. laminariae* was produced using Ion Torrent technology. De novo genome assembly was performed using trimmed high-quality sequencing reads (>Q20). Assembly statistics are presented in Table 1. The total amount of genomic data on average provided more than 15-fold coverage per genome.


**Table 1.** Genome assembly statistics of two *Zobellia* strains.

Genome assemblies were validated by remapping sequencing reads back to contigs using the Bowtie2 program (Table 2). The number of reads aligned exactly one time exceeded 95%, and more than once did not exceed 1%, which reflected the high accuracy of assemblies. It is shown that the combination of the two sequencing technologies enables a higher-quality version of the genome assembly to be obtained. Therefore, the draft genomes of *Z. amurskyensis* and *Z. laminariae* were obtained in sufficient quality for the subsequent bioinformatics analysis.

**Table 2.** Assembly validation metrics.

