*2.5. Study of Channel Modulating Activity*

Since defensins are widely present in animal venoms, also known as toxins with modulating effects on the activity of ion channels [23,29–33], we performed an extensive electrophysiological screening of r-magnificamide against 18 subtypes of voltage-gated potassium and voltage-gated sodium channels (mammalian channels: Kv1.1, Kv1.2, Kv1.3, Kv1.4, Kv1.5, Kv1.6, Kv2.1, Kv3.1, Kv4.2, Kv10.1, hERG, Nav1.2, Nav1.4, Nav1.5, Nav1.6 and Nav1.8; insect channels: Shaker and BgNav1) (Table 4). r-Magnificamide did not reveal ion channel modulating activity, from which it can be surmised, to conclude, that the main biological function of magnificamide is the inhibition of α-amylases.

**Table 4.** Electrophysiological study of r-magnificamide.

