*3.11. Determination of Molecular Weight*

The molecular weight of CamPhoD was determined using gel filtration on a calibrated Superdex G200 PG (GE Healthcare Life Sciences, Amersham Place, Little Chalfont, Buckinghamshire, HP7, 9NA, UK) column (105 × 2 cm) and polyacrylamide gel electrophoresis under denaturing conditions (sodium dodecyl sulfate polyacrylamide gel electrophoresis) using the Lammley method [61].
