3.7.2. Cell Lines and Culture Conditions

22Rv1 and PC-3 cell lines (human prostate cancer cell lines) were purchased from ATCC. Cells were cultured in monolayer in 10% FBS/RPMI media according to the manufacture's protocols and were regularly checked for mycoplasma contamination.

#### 3.7.3. In Vitro MTT- and MTS-Based Drug Sensitivity Assay

The in vitro cytotoxic activity of the isolated compounds was evaluated by the MTT assay (performed as described previously [39]). For glucose uptake assay (3.7.5. Glucose uptake assay, see below) the viability was determined using the MTS assay (performed as described previously [40]). Treatment time was 48 h.
