*4.2. Chemicals and Reagents*

Ech A was received as a gift from G.B. Elyakov, Pacific Institute of Bioorganic Chemistry, Vladivostok, Russia. PPI, SP600125, SB203580, LY294002, and CAL-101 were purchased from Selleck Chemicals (Houston, TX, USA). NAC was obtained from Sigma-Aldrich (St. Louis, MO, USA).

#### *4.3. Immunophenotypic Analysis by Flow Cytometry*

PBMCs, PB-CD34<sup>+</sup> cells, and BM-CD34<sup>+</sup> cells were tested for cell surface antigen expression by immunofluorescence and flow cytometric analysis. Cells were harvested and rinsed with PBS, following which CD34-PE (Miltenyi Biotec, #130-113-179, 1:50), CD38-FITC (Miltenyi Biotec, #130-113-426, 1:50), CD45RA-APC (Miltenyi Biotec, #130-117-742, 1:50), and 7-AAD (eBioscience, Waltham, MA, USA, 3 μL/10<sup>5</sup> cells) were added. Thereafter, cells were incubated at 4 ◦C for 10 min in the dark. Finally, the stained cells were analyzed using a BD AccuriTM C6 (BD Biosciences).
