*3.1. Reagents and Materials*

The reagents of chemical grade qualifications were obtained from Merck (Munchen, Germany), Sigma (Sigma-Aldrich Rus LLC, Moscow, Russia), and Helicon (Moscow, Russia). The molecular biology kits for restriction, ligation, Taq polymerase, and oligonucleotides were from Evrogen (Moscow, Russia) and Thermo Fisher Scientific RU (Moscow, Khimki, Russia); kanamycin was from Sintez (Moscow, Russia). Yeast extract, bactoagar, tripton, and pepton were from Helicon and Dia-M (Moscow, Russia). DNA and protein molecular weight markers were from BioRad (California, USA).
