**3. Layer-by-Layer Approaches for Sca**ff**olds' Fabrication**

A valid alternative to modular μPs-based scaffolds is micro/nanostructured modular scaffolds obtained by layer-by-layer assembly processes, as layers' assembly into 3D modular scaffolds enables the fabrication of geometrically and topographically complex architectures. The layer-by-layer scaffolds' fabrication approaches that are the subject of this review are divided into two groups: discontinuous and continuous processes. Discontinuous processes involve layers' fabrication and assembly in two distinct processing steps. Conversely, the continuous processes steps are almost totally automatized and take place simultaneously. This part of the review will outline and discuss some of the most useful and efficient techniques for layer-by-layer scaffolds' fabrication, highlighting the most promising results in tissue and organ regeneration.
