*2.11. Controls of Specificity*

The specificity of each in situ hybridization reaction was checked in repeated trials using sense NUCB probe at a concentration of 2 ng/μL.

The specificity of each immunohistochemical reaction was checked in repeated trials via pre-absorption of primary antibody Nesf-1 (H-003-24; Phoenix Pharmaceuticals) with homologous antigen Nesf-1 (1–45)/Nesf-1, N-terminal (Human) (003-24; Phoenix pharmaceuticals) (up to 50 mg/mL antiserum in the final dilution). Positive controls were made by sections of rat testis [10]. Internal reaction controls were carried out by substituting primary antisera or secondary antisera with 1× PBS or normal serum in the specific step.
