*2.7. Probe Design*

For the neuroanatomical distribution of NfuNT-6 mRNA, two different DIG-labelled probes were employed: a locked nucleic acid (LNA) probe, and an RNA probe (riboprobe). The LNA probe, unlike the RNA probe, contains an extra bridge which connects the 2 oxygen and 4 carbon locking the ribose in the 3 endo conformation. This conformation significantly increases hybridization properties of the probe.
