*2.6. Statistical Analysis*

Expression levels of NfuNT-6 mRNA were analyzed by the ΔΔCt method and normalized to the housekeeping gene TATA box binding protein (*TBP*): left 5- -CGGTTGGAGGGTTTAGTCCT-3- ; right 5- -GCAAGACGATTCTGGGTTTG-3- ). Fold changes represent the difference in expression levels between young and old age NfuNT-6 cDNAs, respectively, with young and old age TATA-binding protein (TBP) cDNAs. The relative ΔΔ curve threshold was built on fold changes values and *p*-value was <0.01.
