*4.8. Statistical Analysis*

All statistical analyses were performed using the student's *t*-test. The experimental data are presented with the mean standard errors (SE) based on three to six replications. The means were compared by ANOVA, and the significance of the di fferences between group means were calculated by the Bonferroni Post-tests.

**Supplementary Materials:** The following are available online at http://www.mdpi.com/2223-7747/8/5/114/s1. Figure S1: Multiple sequence alignment of ITPK6s. All the data are from RAP-DB (http://rapdb.dna.a ffrc.go.jp/) and Gramene (http://www.gramene.org/). Analysis of ITPK6 sequences from six organisms using BioEdit software. Identical amino acid residues are boxed in same color. Short lines indicate gaps introduced during alignment. The mutant site of OsITPK6 is marked by a redbox. Figure S2. Multiple sequence alignment of *OsITPKs*. All data are from RAP-DB (http://rapdb.dna.a ffrc.go.jp/) and Gramene (http://www.gramene.org/). Analysis of *OsITPK1*, *OsITPK2*, *OsITPK3*, *OsITPK4*, *OsITPK5*, and *OsITPK6* sequences using the BioEdit software. Identical DNA bases are in the same color. Dotted lines indicate gaps introduced during alignment. The target site of *OsITPK6* is marked by a red box.

**Author Contributions:** Q.S. and M.J. planned and designed the research. M.J., Y.L. (Yang Liu), Y.L. (Yanhua Liu), and Y.T. performed the laboratory experiments. M.J., Y.L. (Yang Liu), and Q.S. analyzed the data together. M.J. finished the first draft, which J.H. and Q.S. edited and converted into the final draft. All authors reviewed and approved the final manuscript.

**Funding:** This work was supported by the Zhejiang Provincial S&T Project on Breeding of Agricultural (Food) Crops (Grant No. 2016C02050-2).

**Acknowledgments:** We thank workers in the farms of Zhejiang Zhijiang Seed Tec. Ltd. for taking care of the paddy fields. We also appreciate the assistance in total seed phosphorus and mineral elements measurement of Wu Zhongchang and Xu Jiming from the College of Life Sciences, Zhejiang University, Hangzhou, China.

**Conflicts of Interest:** The authors declare no conflict of interest.
