3.2.4. In Vitro Assessment of Lipomatrix Containing SRO Gastric Resistance (the Test on Lipomatrix Specimens was Performed in Triplicate)

A Semi-Automatic Disintegration Tester (Charles Ischi AG DISI-M) was used to simulate gastric transit of Lipomatix powder as described in Section 3.2.1.2. The dissolution medium was 800 mL of GSF at 37 ◦C (according to Eur. Ph). Three aliquots of Lipomatrix powder as described in Section 3.2.1.2 containing a total amount of 640 mg of SRO was maintained for 90 min in GSF anyone placed in a single chamber of the mentioned device.

#### 3.2.5. Analytical Characterization

#### 3.2.5.1. Differential Scanning Calorimetry (DSC) Analysis

DSC scans were performed by a Mettler-Toledo DSC calorimeter using the following analysis conditions: 25.00–300.00 ◦C, 20K/min and 25.00–125.00 ◦C, 10K/min. The Lipomatrix powder with SRO alone (as described in Section 3.2.1.2) has been processed and each single excipient of which it is composed as well. The main scope of this analysis is to understand the thermal behavior of Lipomatrix powder and if any change in the structure occurs during fusion of the fatty phase and its further rapid cooling during absorption onto the polyol-based powder. The thermal exchanges profile of any component and of the final powder have been determined.
