**5. Conclusions**

The mouse hind limb ischemia model is performed in many variants. No single method for inducing hind limb ischemia that is appropriate for all research questions. The researchers should recognize the variants to be able to choose the most suitable model for their experiments, and they should describe the method and the used anatomical landmarks in order to make comparisons possible among studies.

### **Author Contributions:** Z.A., M.R.d.V., and P.Q. all contributed to conceptualizing, writing and editing the manuscript.

**Funding:** This research forms part of the Project P1.03 PENT of the research program of the Biomedical Materials institute, co-funded by the Dutch Ministry of Economic A ffairs, Agriculture and Innovation.

**Conflicts of Interest:** The authors declare no conflict of interest.
