*4.8. Immunoblotting*

Cell lysates were prepared and subjected to SDS-PAGE followed by western blotting as previously described [28]. Protein band intensities were measured using the Hokawo software (Hamamatsu, Herrsching, Germany) and normalized to the respective β-Actin protein bands, which was used as equal loading control.

### *4.9. Statistical Analysis*

Data are presented as means ± SEM. Statistical analyses were performed with Sigma Plot 10.0. The Student's t-test was used for comparisons between two groups of normal distributed data, rank-sum test was performed for comparisons of two groups of not normally distributed data. The one-way analysis of variance (one-way ANOVA) was performed for multiple comparisons. Differences were considered significant at an error probability level of *p* < 0.05.

**Supplementary Materials:** Supplementary materials can be found at http://www.mdpi.com/1422-0067/20/18/4404/s1.

**Author Contributions:** Conceptualization, supervision and project administration: H.M.; Investigation: Y.H., K.G.G., K.P., H.M.; Methodology: A.P., Y.H., H.M.; Visualization: H.M., Y.H. Writing–original draft preparation: H.M., B.F.K., U.P., A.P.; Funding Acquisition: H.M., Y.H.

**Funding:** This research was funded by *Dr. Kleist-Stiftung* and *Friedrich Baur Stiftung*.

**Acknowledgments:** The authors would like to thank Andrew Kung at Harvard Medical School, Boston, USA for kindly providing us with the lentiviral constructs HIF1-ODD-Luc (FUW-ODDLuc-mCherry) and Ctrl-luc (FUW-wtLuc-mCherry) [16].

**Conflicts of Interest:** The authors declare no conflict of interest and the funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.
