*4.8. Immunohistochemistry*

Cryosections were stained with blue-fluorescent DNA stain DAPI (4,6-Diaminidino-2-phenyllindole-dilactate; Thermo Fisher Scientific, Waltham, MA, USA) and counterstained for αSMA-Cy3 (C6198, Sigma-Aldrich GmbH, Taufkirchen, Germany) or αSMA-FITC (F3777, Sigma-Aldrich GmbH, Taufkirchen, Germany) and CD68 (MCA1957A448T, AbD Serotec, BioRad, Feldkirchen, Germany).

### *4.9. Statistical Analysis*

All statistical analyses were supported by GraphPad software PRISM5 for Mac (GraphPad Software, La Jolla, CA, USA), JMP for Mac (Version 9.0.12010 SAS Institute Inc., Heidelberg, Germany), Image J (National Institutes of Health, Bethesda, Maryland, USA) and Microsoft Excel for Mac (Version16.15, Microsoft, Redmond, Washington, USA). Comparisons between groups were based on unpaired student's t-test. One-way or two-way ANOVA were used to determine differences between the means of three or more independent groups as indicated. Data were reported as standard error of mean (SEM). Data deviations were considered to be statistically significant differences at *p* < 0.05.
