*4.6. Intervention*

The three training conditions were (1) resistance training during BFR at 30% of the 1RM, (2) resistance training without BFR at 30% of the 1RM, and (3) resistance training without BFR at 70% of the 1RM. The possible sequences of the conditions to be performed were randomly assigned to the participants in a balanced frequency. Resistance training comprised knee extensors and flexors on a combination training device (Schnell M3 Diagnosis, Peutenhausen, Germany). In conditions (1) and (2), resistance training with 30% of 1RM was adopted, and each exercise period consisted of 75 repetitions divided into 4 sets with a rest period of 90 s. In contrast, in condition (3), the resistance training was performed at 70% of the 1RM; here, 30 repetitions divided into 3 sets with a break of 90 seconds between sets. During condition (1), blood pressure cuffs inflated to 300 mm Hg were applied to both legs. The exercise protocols have been used previously in other studies and have been classified as low risk [44]. The washout phase between test days was at least 7 days. On each test day, a standardized control condition ("do-nothing" phase) was performed for 20 min before the test condition.
