*2.3. Flow Cytometry*

Flow cytometry measurements were performed using BD LSRFortessa (BD Biosciences) and analyzed on BD FACSDiva v8.0 and FlowJo-v10 software (BD Biosciences).

### *2.4. Immunohistochemical Analysis*

Images were acquired using a Zeiss epifluorescent microscope (Carl Zeiss Microscopy GmbH, Munic, Germany) and analyzed using AxioVisionRel 4.8 software (Carl Zeiss Microscopy GmbH).
