**Anna Wajs-Bonikowska 1, Janusz Malarz <sup>2</sup> and Anna Stojakowska 2,\***


Received: 31 October 2019; Accepted: 2 December 2019; Published: 3 December 2019

**Abstract:** *Carpesium divaricatum* Sieb. and Zucc. has long been used both as traditional medicine and seasonal food. The most extensively studied specialized metabolites synthesized by the plant are sesquiterpene lactones of germacrane-type. Low-molecular and volatile terpenoids produced by *C. divaricatum*, however, have never been explored. In this work, compositions of essential oils distilled from roots and shoots of *C. divaricatum* plants, cultivated either in the open field or in the glasshouse have been studied by GC-MS-FID supported by NMR spectroscopy. The analyses led to the identification of 145 compounds in all, 112 of which were localized in aerial parts and 80 in roots of the plants grown in the open field. Moreover, remarkable differences in composition of oils produced by aerial and underground parts of *C. divaricatum* have been observed. The major volatiles found in the shoots were: α-pinene (40%), nerol (4%) and neryl-isobutyrate (3%), whereas predominant components of the root oil were 10-isobutyryloxy-8,9-epoxythymyl-isobutyrate (29%), thymyl-isobutyrate (6%) and 9-isobutyryloxythymyl-isobutyrate (6%). In the analyzed oils, seventeen thymol derivatives were identified. Among them eight compounds were specific for roots. Roots of the plants cultivated in the glasshouse were, in general, a poor source of essential oil in comparison with those of the plants grown in the open field. Chemophenetic relationships with other taxa of the Inuleae-Inulineae were also briefly discussed.

**Keywords:** alpha-pinene; *Carpesium divaricatum*; Inuleae; monoterpenoids; thymol derivatives
