**3. Results**

#### *3.1. Animal and Transcriptome Sequencing*

After the Ashmead animals (three females and one male) were maintained with oriental fruit flies, which were fed guava (G), mango (M), and carambola ©, the antenna, head, breast, abdomen, and feet tissues were mixed together for RNA extraction and transcriptome sequencing. After data cleaning, we obtained a total of ~622.32 million reads (~69.15 million reads on average) and assembled 24,201 to 34,302 genes using Trinity for all the samples (Table 1). After similar genes/transcripts were clustered and merged, we finally obtained 162,621 Unigenes for the Ashmead transcriptome, with an average length of 1425.14 bp. The N50, GC content, and size of the transcriptome were calculated as 3572, 41.88%, and ~231 M, respectively (Table 1). Length distribution analysis showed that 53.05% of the total transcripts were longer than 500 bp and 11,101 transcripts (6.83% of the total transcripts) were longer than 5000 bp (Figure 1A). Last, we used BUSCO to evaluate the completeness of the assembled Unigenes and the results showed 99.6% of the assembled Unigenes were complete. In detail, out of the 255 evaluated BUSCOs in the dataset, 254 were complete, including 228 duplicated and 26 single-copy BUSCOs.

