*2.6. Statistical Analysis*

For q-PCR, transcript level ratios were compared between strains (or body fractions) using Relative Expression Software Tool [57] (REST, REST-MCS beta software version 2) with 2000 iterations. This is based on the probability of an e ffect as large as that occurring under the null hypothesis (no e ffect of the treatment), using a randomization test (Pair-Wise Fixed Reallocation Randomization Test) [60]. For behavior and EAG, statistical analyses were performed using XLSTAT software. Within each genotype, the CI directed towards each sex target was compared with a Student's *t*-test. Since CIf and CIm are linked, they were compared between strains using ANOVA and LSD-Fischer post-hoc tests (sex target/strain genotype). Locomotor activity values were normally distributed and compared with a Mann-Whitney test (*p* < 0.05). The di fference of EAG amplitude between stimulation was compared using a Wilcoxon bilateral test. EAG comparison between strains was performed with a Kruskal-Wallis test (*p* < 0.05).
