*2.6. Data Analysis*

For each experiment, the number of labeled OB cells per section was quantified along the rostrocaudal axis within the OB (32–40 sections per animal). Cells were counted using the manual cell counter plug-in of ImageJ software and the percentage of those cells, located in specific areas, was calculated. The study of 26,685 labeled interneurons in the OB was considered in this approach. For statistics, GraphPad Prism 6.0 (GraphPad, San Diego, CA, USA) was used, and the statistical significance between two groups was assessed with two-tailed unpaired Student's *t*-tests. For multiple comparison study, one-way analysis of variance (ANOVA) was used. A confidence interval of 95% (*p* < 0.05) was used to determine statistically significant values. Critical values of \* *p* < 0.05, \*\* *p* < 0.01, and \*\*\* *p* < 0.001 were adopted to determine statistical differences. Graphs were obtained using GraphPad Prism and CorelDRAW Graphic Suite 2018 (Corel Corporation, Ottawa, Canada).
