*2.9. Statistical Analysis*

All parameters in the study were distributed normally. Data are expressed as mean ± SEM. Di fferences were tested by unpaired t-test (Figure 1, Figure 2 and Figure 3) or by using multiple t-tests employing two-stage linear step-up procedure of Benjamini, Krieger and Yekutieli, with a false discovery rate set to 1% (Figure 4 and Figure 5). Each group was analyzed separately without the assumption of consistent standard deviation. *p* < 0.05 values were considered statistically significant. Statistical analysis was performed with GraphPad Prism 7.03 (GraphPad Software, Inc., San Diego, CA, USA).
