*2.5. Lectin Cytochemistry*

Paraformaldehyde-fixed eyes (as described above) were processed for paraffin embedment. Paraffin sections of mouse eyes were then incubated (45 min at room temperature) with biotinylated Concanavalin-A (ConA, B-1005; Vector Laboratories; 1:200 dilution in PBS), followed by incubation with AlexaFluor®-488 conjugated streptavidin (Thermo Fisher Scientific; 1:500 dilution in PBS) and AlexaFluor®-647-conjugated peanut agglutinin (PNA, L32460; Thermo Fisher Scientific; 1:250 dilution in PBS), with or without pre-treatment (37 ◦C, overnight) with peptide:N-glycosidase F (PNGase-F, 200 U, P0704S; New England Biolabs, Inc., Ipswich, MA, USA). Sections were DAPI-stained and mounted using Vectashield mounting media, and digital images obtained using scanning laser confocal microscopy as described above [16].
