**4. Conclusions**

In this work, novel fluorescence imprinted polymers were synthesized via an oil-in-water Pickering emulsion polymerization, in which the whole bacteria was used as the template, and a bacteria-NAC-QD complex was used as the stabilization particle at the oil-water interface. The MIPs provided a fast mass transfer rate and excellent specific adsorption for *L. monocytogenes*. In addition, the obtained MIPs offered sensitive and visual detection of *L. monocytogenes* in milk and pork samples based on changes in their fluorescence color under UV lamp excitation. We believe this strategy will promote the development of fluorescence bacteria imprinted technology and have the potential to be extended to the analysis of other foodborne pathogens.

**Author Contributions:** X.Z., Y.C.; investigation, Y.C.; writing—original draft preparation, X.Z.; writing—review and editing, J.W. (Junying Wang); supervision, J.W. (Junying Wang), J.W. (Junping Wang); funding acquisition.

**Funding:** This research was funded by the National Key R&D Program of China (grant no. 2016YFD0401202) and the Special Project of the Tianjin Innovation Platform (grant no. 17PTGCCX00230).

**Acknowledgments:** The authors are grateful for language help provided by LetPub.

**Conflicts of Interest:** The authors declare no conflict of interest.
