*2.3. Bionic Microfluidic Device Design and Visualization of Injected Erythrocyte Trajectories*

The bionic microfluidic channel was designed using AutoCAD software and fabricated through standard lithographic method [17]. The microfluidic device was composed of polydimethylsiloxane (PDMS) and a glass plate, which is one layer with the height of 20 μm, and the detailed channel parameters are shown in Figure 1. After exposed to stable shear stress on a rheometer platform for 10 min, the whole blood sample was collected and injected into the inlet hole of the microchannel with the flow rate of 0.12 mL/min. The visualization of injected flowing erythrocyte trajectories in the microchannel were captured using a high-speed camera (M710, Phantom Co., New Jersey, USA) under the bright field provided by an inverted microscope (Leica DME6000 B, Leica Co., Wetzlar, Germany). The video analysis was processed using ImageJ software.
