*2.6. Immunohistochemistry*

Immunohistochemistry was performed on formalin-fixed and paraffin-embedded (FFPE) liver sections for α-smooth muscle actin (α-SMA) (clone ASM-1/1A4; Sigma-Aldrich, Taufkirchen, Germany), platelet derived growth factor-β (PDGFR-β) (clone 42G12; Abcam, Cambridge, UK), and S100 calcium binding protein A6 (S100A6) (clone EPNCIR121; Abcam). All primary antibodies were diluted 1:100. For immunofluorescence, secondary goat anti-mouse Cy5 (Abcam) and goat anti-rabbit Al488 (Abcam) were used at a dilution of 1:200. Nuclei were stained with DAPI (Sigma-Aldrich, Taufkirchen, Germany). Micrographs were taken using an Axio Observer Z1 equipped with an Axio Cam MR (Zeiss, Oberkochen, Germany)
