*2.1. Materials*

Dulbecco's modified Eagle Medium (DMEM) was from Gibco (Thermo Fisher Scientific, Waltham, MA, USA). FCS was acquired from Perbio Science (Bonn, Germany). Pencillin, streptomycin, and amphotericin B were purchased from Gibco (Thermo Fisher). Taurolithocholic acid (TLC) and forskolin were purchased from Sigma-Aldrich (St. Louis, MO, USA). DMSO (Dimethyl sulfoxide) was acquired from Carl Roth (Karlsruhe, Germany). The TGR5 agonist RO5527239((R,E)- 1-(4-(3-(hydroxyimino)-3-(2-methylpyridin-4-yl)-1-o-tolylpropyl)phenyl)piperi-dine-4-carboxylic acid) was kindly provided by F. Hoffmann-La Roche, (Basel, Switzerland) [27]. Pronase P and Dnase I for isolation of murine HSCs were also from F. Hoffmann-La Roche. Pronase E for isolation of HSCs was purchased from Sigma-Aldrich and collagenase type I from Worthington (Lakewood, WA, USA). Collagenase type I for isolation of LSECs was from Sigma-Aldrich.
