*2.10. Measurement of Portal Pressure*

TGR5 KO and WT mice on chow or LCA diet (1%, 84 h) were anesthetized and the portal vein was cannulated. Invasive blood pressure measurement was performed using the pressure transducer P75 and amplifier from Hugo Sachs Electronics (Harvard Apparatus GmbH, March, USA). Additionally, 6-week-old WT mice were anesthetized and cannulation of portal vein for blood pressure measurement was done. Mice livers were then perfused with either control HBSS solution or a solution containing 10 μM TGR5 agonist for 15 min. Subsequently, increasing concentrations of ET-1 (5, 10, and 15 nM) were added to the perfusion media for 15 min each. Perfusion pressure in the portal vein was measured constantly over the whole time course. Mean portal pressure was taken for each condition of each mouse. Portal pressure under control conditions (t = 0) of HBSS buffer perfusion supplemented with DMSO (=vehicle) were set to 1.0.
