*2.5. Morphometric Analysis*

As many liver lobules as possible were photographed in two Sirius Red/Fast Green-stained sections of each mouse (magnification 200×, blinded for groups). Images suitable for analysis were identified following predefined exclusions criteria and the red stained area was quantified using the color threshold tool in ImageJ, version 1.51 [45]. To quantify the stained area in CD45 immunohistochemical sections, as many non-overlapping high-power fields as possible were photographed using a Biozero BZ-8000 microscope (magnification 200×, Keyence, Osaka, Japan). Suitable images were converted to 8-bit grey scale and the threshold tool in ImageJ was used to quantify the stained area.
