*2.3. Human LX-2 CELLS*

The human HSC (hHSC) line, LX-2, (a kind gift from Dr Scott Friedman, Icahn School of Medicine at Mount Sinai, New York, NY, USA) was cultured in DMEM/10% FBS. Cells were seeded at 10<sup>7</sup> cells/mL and allowed to grow to 90% confluency. For harvesting of EVs, spent medium was removed and replaced with serum-free DMEM which was then collected 2 days later.
