*2.11. Hematoxylin Eosin- and Picro-Sirius Red Staining*

Liver samples were fixed in 4% paraformaldehyde, embedded in paraffin, and sliced into sections of 5 μm. Following rehydration, liver slides were stained in hematoxylin (Hematoxylin Solution Gill No.3, Sigma Aldrich) for 1 min and in eosin for 3 min. Alternatively, slides were treated with the Picro-Sirius Red staining kit according to the manufacturer's instructions (Polysciences, Warrington, FL, USA). After dehydration, slides were mounted with VectaMount (Vectorlabs, Burlingame, CA, USA). Images were acquired on a Zeiss AxioLab A1 Microscope (Carl Zeiss, Jena, Germany) with 63×, 40×, 20×, and 10× objectives.
