2.4.2. Zeta Potential

The zeta potential was determined by applying an electric field across the samples, and the value of the zeta potential was obtained by measuring the velocity of the electrophoretic mobility of the particles using the laser Doppler anemometry technique. The measurements were performed in triplicate for each sample at 25 ◦C using a Malvern Zetasizer Nano ZS (Malvern Instruments, Worcs, UK). Milli-Q ® water was used to dilute the NLC to a proper concentration. The zeta potential was calculated using the Helmholtz-Smoluchowsky equation included in the software of the system. Values are presented as the mean of triplicate runs per sample.
