*2.5. Total Phenolic Content (TPC)*

The TPC was assessed using the Folin-Ciocâlteu method [21] with a SPECTROstar Nano Multi-Detection Microplate Reader in 96-well plates (BMG Labtech, Ortenberg, Germany). Briefly, a mixture solution consisting of 20 μL of extract, 100 μL of Folin-Ciocâlteu reagent, and 80 μL of sodium carbonate (Na2CO3, 7.5% *w*/*v*) was homogenized and incubated at room temperature in the dark (30 min). The TPC was expressed as gallic acid equivalents (GAE) in milligrams per gram dry weight (dw) of plant material (mg GAE/g dw plant material) after reading the absorbance of the samples at 760 nm.
