2.2.3. Tocopherols

Tocopherols were determined in the lyophilized samples following a procedure previously described by Dias et al. [22]. The separation of compounds was performed using a high performance liquid chromatography system (Knauer, Smartline system 1000, Berlin, Germany) coupled to a fluorescence detector (FP-2020; Jasco, Easton, PA, USA) programmed for excitation at 290 nm and emission at 330 nm, using the internal standard (IS; tocol, Matreya, Pleasant Gap, PA, USA) method for quantification. After separation, the compounds were identified by comparison with authentic standards. The quantification was based on the fluorescence signal response of each standard, using the internal standard method. The results were expressed in μg per 100 g of fresh weight (fw).
