*2.4. Conventional Heat Reflux Extraction (HRE)*

A comparative study between the HRE and UAE was carried out to estimate the efficiency of the UAE process established in this work. The HRE of TF from *P. cretica* was performed under the optimized UAE conditions with slight modifications. In brief, 5.0 g of the pre-prepared sample was extracted three times under reflux with 170 mL of 57% ethanol at 75 ◦C, each time for 2 h. After the HRE, the extract was processed as the method described in Section 2.3.1.
