*2.8. Essential Oil Yield and Composition*

Aerial parts of *S. perfoliata* plants were harvested and three biological samples (pooled of three individual plants/sample) were air-dried at 42 ◦C in an oven, chopped and were hydro-distilled for 3 h, using a Clevenger apparatus for essential oil (EO) extraction. The EOs were analyzed by Gas Chromatography-Mass Spectrometry (GC/MS) (Shimadzu GC/MSQP-2010 Plus, Tokyo, Japan) and their constituents were determined as described previously [25].
