2.2.1. Standards and Reagents

Acetonitrile 99.9% of HPLC grade was from Fisher Scientific (Lisbon, Portugal). Ellipticine was purchased from Sigma-Aldrich (St. Louis, MO, USA), as also were acetic acid, sulforhodamine B (SRB), trypan blue, trichloroacetic acid (TCA), and Tris-(hydroxymethyl)aminomethan (TRIS). Phenolic compound standards were from Extrasynthèse (Genay, France). RPMI-1640 medium, fetal bovine serum (FBS), Hank's balanced salt solution (HBSS), l-glutamine, nonessential amino acids solution (2 mM), penicillin/streptomycin solution (100 U/mL and 100 mg/mL, respectively), and trypsin-EDTA (ethylenediaminetetraacetic acid) were from Hyclone (Logan, UT, USA). All other chemicals were of analytical purity and obtained from common suppliers. Water was treated via the purification system Milli-Q water (TGI Pure Water Systems, Greenville, SC, USA).
