2.7.1. DPPH Radical Scavenging Assay

The DPPH antioxidant capacity was determined according to the method described in Mocan et al. [32], with some modifications, by using a SPECTROstar Nano microplate reader (BMG Labtech, Offenburg, Germany). Each of the 96 wells consisted of 30 μL of sample solution (in an appropriated dilution) and a 0.004% methanolic solution of DPPH. After 30 min of incubation in the dark, the absorbance of the sample was read at 515 nm. Results were expressed as Trolox equivalents per gram of dry weight herbal extract (mg TE/g dw herbal extract).
