2.2.5. Fatty Acids

To determine these compounds, and after performing the extraction and derivatization procedures, the analysis was achieved through a DANI model GC 1000 instrument equipped with a split/splitless injector, a flame ionization detector (FID) at 260 ◦C and a Zebron–Kame column (30 m × 0.25 mm ID × 0.20 μm df, Phenomenex, Torrance, California, USA) [8]. The fatty acids identification was possible by comparison of the relative retention times of fatty acid methyl ester (FAME) peaks from samples with standards. The obtained data was analyzed using the Clarity DataApex 4.0 Software and expressed in relative percentage of each fatty acid.
