3.1.5. Peroxiredoxins Expression

Figure 6 represents the expression of peroxiredoxins, a ubiquitous family of thiol-dependent peroxidases involved in peroxide detoxification and redox signaling [27,28]. Prx1 was increased in a dose-dependent manner over control cells (Figure 6A,B). Interestingly, mitochondrial Prx3 reached higher levels at the lower urolithin concentration tested (Figure 6C,D). Urolithin A (1 and 2 μM) treatments displayed significant differences in Prx1 expression in N2a cells over control cells (*p* < 0.05; *p* < 0.01).

**Figure 6.** Urolithin A-induced cytoprotection of Neuro-2a cells is mediated by induction of peroxiredoxin 1 and 3 (Prx1, Prx3). Prx 1 (**A**,**B**) and Prx 3 (**C**,**D**) expressions were determined by Western blot in 10 μg of protein extract and expressed as Prx densitometry. Ponceau red staining of membranes prior to blotting was performed to further check protein transfer and loading. Note: # *p* < 0.05 versus Control; ## *p* < 0.01 versus Control
