2.4.2. DPPH Radical Scavenging Activity

The antiradical activity of HI extracts against the free radical DPPH was measured using a method previously described [19]. In a 96-well plate, 30 μL of sample solution was mixed with a 0.004% methanol solution of DPPH, and then incubated in the dark for 30 min. The absorbance was measured at 517 nm against a solvent blank. Trolox was used as a reference standard and the results were expressed as TE per dw extract (mg TE/g dw extract). This assay was performed only on the richest polyphenolic HI extract.
