*2.5. Trolox Equivalent Antioxidant Capacity*

The Trolox equivalent antioxidant capacity (TEAC) was determined according to the assay reported by Re et al. [28]. ABTS•<sup>+</sup> was generated and diluted as in the original description. Next, solutions of ABTS•<sup>+</sup> (2 mL) and amaranth extracts (20 μL with a concentration of 2.5 mg/mL) were vortexed and heated at 30 ◦C (TH-24 block heater, Meditherm, Warsaw, Poland) within 6 min. After incubation, absorbance was measured at 734 nm (DU-7500 spectrophotometer). The results were expressed as μmol Trolox equivalents (TE) per g of extract or per g of FM of plant.
