2.2.4. Tocopherols

After an extraction procedure following the recommendations of Pereira et al. [9], the tocopherols were injected in a Knauer Smartline system 1000 (HPLC, Berlin, Germany) with a fluorescence detector (FP-2020; Jasco, Easton, USA). Separation was achieved using a Polyamide II normal-phase column (250 mm × 4.6 mm, 5 μm, YMC Waters, Milford, MA, USA), with an isocratic elution using n-hexane and ethyl acetate (70:30, *v*/*v*) at a flow rate of 1 mL/min, operating at 35 ◦C. The internal standard method was applied, and the results are expressed in mg per 100 g of fresh fruit.
