*2.10. Statistical Analysis*

Experiments were performed in triplicate in three independent experiments, unless otherwise stated. Data were statistically analyzed using IBM SPSS v.21 for analysis of variance (ANOVA) and presented as means ± standard deviation (SD). Duncan's multiple range test (DMRT) was used for mean comparisons when significant differences were detected (*p*-value < 0.05). The absolute 50% inhibitory concentration (IC50) was calculated using the GraphPad Prism version 4.00 for Windows, GraphPad Software (La Jolla, CA, USA).
