**4. Conclusions**

*Sideritis perfoliata* has a high protein and antioxidant content due to the presence of flavonoids and phenolics. Moreover, we revealed the wrinkle reducing potential of *S. perfoliata* as it showed noteworthy elastase inhibition. Traditionally, *S. perfoliata* is used for the treatment of wounds which could be attributed to the antibacterial and antioxidant activity reported in this study. The ethanol extract showed moderate toxicity towards the non-cancerous cell lines, whereas low toxicity was noted against the cancerous cell lines. However, the most promising anticancer activity was noted against human liver cancer cells.

**Author Contributions:** A.C. performed chemical analysis, A.C. and I.L. composition and wrote part of the original draft; I.L. performed chemical analysis, data curation, methodology and coordinated the South African team manuscript draft preparation; B.F., A.B.V.S., D.T., M.N.d.C., C.B.O., D.B. performed chemical analysis and analysis of results; N.L. administered and supervised the project, reviewed and edited the final manuscript; N.T. conceived and designed the research, administered and supervised the project, obtained funding, reviewed and edited the final manuscript.

**Funding:** The research performed at the University of Pretoria was funded by the National Research Foundation (NRF) and the University of Pretoria. Funding for publication was granted by the Cyprus University of Technology Open Access Author Fund.

**Conflicts of Interest:** The authors declare no conflict of interest.
