2.5.1. Antioxidant Activity

The lipid peroxidation inhibition was evaluated trough the TBARS assay, which mainly consists of using porcine (*Sus scrofa*) brain homogenates and the antioxidant potential was measured by the decrease in thiobarbituric acid reactive substances (TBARS) as described by Pereira et al. [12]. These results were expressed in μg/mL corresponding to the EC50 value (sample concentration providing 50% of antioxidant activity). Another antioxidant assay applied was the anti-haemolytic activity of the obtained extracts, evaluated through the oxidative haemolysis inhibition assay (OxHLIA), according to Lockowandt et al. [13]. These results were expressed in μg/mL corresponding to the IC50 value, which is the concentration capable of promoting a Δt haemolysis delay of 60 and 120 min. Trolox was used as a positive control in both of the assays.
