2.2.2. Free Sugars

These sugars were extracted from the lyophilized fruit and were further injected in HPLC equipment (Knauer, Smartline system 1000, Berlin, Germany) coupled to a refractive index detector (RI detector, Knauer Smartline 2300) as described by Barros et al. [8]. Separation was achieved with a Eurospher 100-5 NH2 column (250 mm × 4.6 mm, 5 μm, Knauer), with an isocratic elution using acetonitrile/deionized water (70:30, *v*/*v*) at a flow rate of 1 mL/min, operating at 35 ◦C. The internal standard method was applied, and the results were expressed in g per 100 g of fresh fruit.
