*2.8. Fe2*<sup>+</sup> *Chelating Ability*

The ability of amaranth extracts to chelate ferrous ions was determined by a method with ferrozine [31] that was modified to be performed with multi-well plates [32]. Extract solution in water (0.25 mg/mL), 0.4 mM FeCl2 × 4H2O and 5 mM ferrozine were mixed in a ratio of 10:1:2 (*v*/*v*/*v*). After 10 min, absorbance was measured at 562 nm using an Infinite M1000 microplate reader (Tecan, Männedorf, Switzerland). The chelating ability was expressed as percentage of Fe2<sup>+</sup> bound.
