2.2.6. Fatty Acids

Fatty acids were determined by gas-liquid chromatography with flame ionization detection (GC-FID; DANI1000, Contone, Switzerland), after the extraction and derivatization procedures previously described by Obodai et al. [24]. Fatty acid identification and quantification were performed by comparing the relative retention times of fatty acid methyl ester (FAME) peaks of the tested samples with commercial standards (fatty acid methyl esters (FAMEs) reference standard mixture 37, Sigma-Aldrich, St. Louis, MO, USA). Results were expressed as the relative percentage for each detected fatty acid, using CSW 1.7 software (Data Apex 1.7, Prague, Czech Republic).
