2.2.3. Organic Acids

Organic acids were extracted from the lyophilized fruit and analyzed via ultra-fast liquid chromatography (UFLC) coupled to photodiode array detector (PDA), employing a Shimadzu 20A series UFLC (Shimadzu Cooperation), according to Barros et al. [8]. Separation was achieved using a SphereClone reverse phase C18 column (250 mm × 4.6 mm, 5 μm, Phenomenex, Torrance, CA, USA), with an isocratic elution using sulphuric acid (3.6 mM) at a flow rate of 0.8 mL/min, operating at 35 ◦C. The results were expressed in g per 100 g of fresh fruit.
