*2.3. Extraction*

Plant samples were lyophilized (FreeZone Freeze Dry System, Labconco, Kansas City, MO, USA) and ground. Three-gram portions of materials were suspended in 30 mL of 80% (*v*/*v*) methanol. The closed bottles containing suspensions were shaken for 15 min in a water bath (SW22, Julabo, Seelbach, Germany) at 65 ◦C. After filtration, the residues were extracted twice more. Combined filtrates for each sample were dried by evaporation of methanol under vacuum at 50 ◦C (Rotavapor R-200, Büchi Labortechnik, Flawil, Switzerland) and lyophilization of remaining water. Yield of extraction (%) was calculated on the basis of weight of plant sample and dry extract.
