*2.6. Cell Culture*

The HT-22 cell line was procured from Medifron (Seoul, Korea), and HT-22 cells were maintained in DMEM medium with 10% FBS, 100 units/mL of penicillin, and 100 μg/mL of streptomycin at 37 ◦C in a humidified atmosphere of 5% CO2. Passages three to 11 of the cells were used in this study. All the in vitro studies included a vehicle control group (0.1% dimethyl sulfoxide).
