2.4.1. Determination of Lipid Peroxidation

Lipid peroxidation was determined following a method reported previously [22]. Briefly, brains isolated from sacrificed mice were homogenized in ice-cold 1× PBS, and then the homogenates were centrifuged (17,000× *g* at 4 ◦C) for 10 min. The supernatant (100 μL) was mixed with 50 μL of 20% aqueous trichloroacetic acid and 100 μL of 0.67% aqueous thiobarbituric acid, boiled for 10 min, and then centrifuged (60× *g*, room temperature (RT) for 30 min. The absorbance of the supernatant was measured at 532 nm using a microplate reader (DU650, Beckman Coulter, Brea, CA, USA).
