*2.9. Scavenging of the DPPH Radicals*

The DPPH• scavenging activity of amaranth extracts was evaluated by the method of Brand-Williams et al. [33]. Extract solutions in methanol over a range of concentrations from 2–10 mg/mL were prepared. To 100 μL of these solutions, 0.25 mL of 1 mM DPPH and 2 mL of methanol were added. After 20 min, absorbance of the mixtures was read at 517 nm. The curves of absorbance values vs. concentration of samples (mg/assay) were plotted. Additionally, EC50 values defined as concentration of extract (mg/mL reaction mixture) needed to scavenge the 50% of initial DPPH• were evaluated.
