*2.7. Antidiabetic (Glucosidase inhibitory) Assay*

The α-glucosidase inhibitory assay was measured according to method described previously [20,21]. In brief, 50 μL of sample diluted in 50 μL 100 mM-phosphate buffer (pH 6.8) in a 96-well microplate, were mixed with 50 μL yeast α-glucosidase for 10 min before 50 μL substrate (5 mM, p-nitrophenyl-α-D-glucopyranoside prepared in same buffer) were added. The release of p-nitrophenol was measured at 405 nm, 20 min after incubation. The blanks for test samples were prepared, and acarbose was used as a standard inhibitor, the results being expressed as IC50 or percentage of inhibition (where the

sample was not enough active to be able to calculate an IC50 value for a tested concentration of 8 mg/mL) using the following formula:

$$\text{Inhibition (\%)} = \text{[(Abs}\_{\text{control}} - \text{Abs}\_{\text{sample}}) / \text{Abs}\_{\text{control}}] \times 100 \tag{1}$$
