2.7.3. FRAP Assay

The FRAP assay was assessed using the method described in Damiano et al. [33] with slight modifications. The FRAP reagent was prepared by mixing ten volumes of acetate buffer (300 mM, pH 3.6), one volume of 2,4,6-tris(2-pyridyl)-*s*-triazine (TPTZ) solution (10 mM TPTZ in 40 mM HCl), and one volume of FeCl3 solution (20 mM FeCl3·6H2O in 40 mM HCl). After mixing the samples with the reagent (25 μL sample and 175 μL FRAP reagent), the samples were incubated in the dark for 30 min at room temperature and the absorbance was measured at 593 nm in 96-well plates. The final results were expressed as milligrams of Trolox equivalents (TE) per milligram of extract (mg TE/g extract).
