Cell Culture

The normal human gingival fibroblasts (HGF) (CLS Cell Lines Service, Eppelheim, Germany), and the cancerous cell lines A549 (human lung adenocarcinoma) and T47D-KBluc (human breast cancer) (ATCC, Manassas, United States of America) were maintained in Dulbecco's modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS) on culture flasks at 37 ◦C in a humidified incubator with 5% CO2 supplementation. The medium was changed every two to three days and the cells were subcultured once they reached 70%–80% confluence.
