*2.3. Characterization of Extract*

The determination of the polyphenol content was performed spectrophotometrically, using the Folin–Ciocalteu method [20]. The total polyphenol content (TPC), expressed in mg gallic acid (GAE)/g plant material, was calculated based on the absorbance read with the spectrophotometer at 765 nm, taking into account the calibration curve (concentration range between 0 and 200 mg/L) of the standard gallic acid solution.
