*2.3. Ethanolic Extract Preparation*

Aerial parts (leaves and stems/shoots) of *S. perfoliata* were air dried at 42 ◦C in an oven and then milled to a fine powder. The plant material was then macerated with pure ethanol (1:2.5 v/v) in a shaking incubator at 160 rpm, in glass bottles. After 72 h of shaking, the material was filtered using filter paper (Whatman No. 1, Merck, Darmstadt, Germany). The remaining ethanol was removed using a rotary evaporation unit, until complete dryness.
