2.2.5. Organic Acids

The lyophilized samples were extracted using a methodology previously described and optimized by the authors [23]. The analysis was performed by Ultra-Fast Liquid Chromatography coupled with a Diode-Array Detector (UFLC-DAD; Shimadzu 20A series UFLC, Shimadzu Corporation, Kyoto, Japan). Compounds were identified and quantified by comparison of the retention time, spectra and peak area, recorded at 215 nm, with those obtained from commercial standards. The results were recorded and processed using LabSolutions Multi LC-PDA software (Shimadzu Corporation, Kyoto, Japan) and were expressed in g/100 g fw.
