Preparation of Extract Solutions

A 100 mg/mL stock solution was prepared in dimethyl sulfoxide (DMSO) from lyophilized extract. The stock solution was further diluted in DMSO to obtain working solutions of 0.25, 6.25, 12.5, 18.75, 25, 37.25, 50, and 75 mg/mL. These working solutions were then used to obtain the desired concentrations, ranging from 400 to 1 μg/mL, in the cell culture medium.
