2.4.1. TEAC Assay

The antiradical activity of HI extracts was assessed according to the TEAC assay previously described [17]. Briefly, 20 μL of the sample was mixed with 200 μL of ABTS radical solution, incubated for 6 min, and the absorbance of the mixture was measured at 760 nm. The scavenging activity against ABTS radical cation was calculated and used to plot the Trolox calibration curve. The AA according to this assay was expressed as Trolox equivalents (TE) per dw involucre (mg TE/g dw). This assay was used during the screening phase of the study for the evaluation of AA of all HI extracts obtained within the experimental plan in order to optimize the extraction method.
