*2.1. Materials*

Antibiotics, FBS, 0.25% trypsin-EDTA, and 2,7-dichlorofluorescein diacetate (DCFDA) were procured from Invitrogen (Carlsbad, CA, USA). Dulbecco's modified eagle medium (DMEM), 1× PBS, and 1× Tris buffered saline (TBS) were purchased from Welgene, Inc. (Gyeongsan, Gyeongbuk, Korea). EZ-Cytox cell viability assay kit was obtained from DAEIL Lab (Seoul, Korea). DIM, K252a (a TrkB inhibitor), and MK-2206 (a specific Akt inhibitor) were procured from Cayman Chemical Company (Ann Arbor, MI, USA). Specific antibodies against cytochrome c (ab90529), glutamate-cysteine ligase catalytic subunit (GCLC, ab53179), and NAD(P)H quinone oxidoreductase-1 (NQO-1, ab34173) were purchased from Abcam, Inc. (Cambridge, UK). A specific antibody against BDNF (sc-65514) was obtained from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA). Specific antibodies against apoptosis-inducing factor (AIF, #4642), B-cell lymphoma 2 (Bcl-2, #3498), Bcl-2-associated X protein (Bax, #2772), cleaved caspase-3 (#9661), heme oxygenase-1 (HO-1, #70081), NF-E2-related factor-2 (Nrf2, #12721), phospho-Akt (#9271), phospho-cAMP response element-binding protein (CREB, #9198), phospho-TrkB (#4619), and β-actin (#4970) as well as a horseradish peroxidase-conjugated IgG secondary antibody were procured from Cell Signaling Technology (Beverly, MA, USA). Muse Annexin V and Dead Cell Assay Kits were purchased from Merck Millipore, Inc. (Darmstadt, Germany). All other chemicals which were used in this study were analytical grade.
