*2.5. Statistical Analysis*

All analyses were performed in triplicate, and the results were presented as mean ± standard deviation. All statistical tests were performed at a 5% significance level using SPSS Statistics software (IBM SPSS Statistics for Windows, Version 22.0, IBM Corp., Armonk, NY, USA:). The fulfilment of the one-way analysis of variance (ANOVA) requirements, specifically the normal distribution of the residuals and the homogeneity of variance, was tested by means of the Shapiro Wilk's and Levene's tests, respectively. Depending on the homoscedasticity, the dependent variables were compared using Tukey's honestly significant difference (HSD; when homoscedastic, *p* > 0.05) or Tamhane's T2 multiple comparison (when heteroscedastic, *p* < 0.05) tests.

Differences between two samples were analysed by applying a two-tailed paired Student's *t*-test; significant differences were considered when the *p*-value was lower than 0.05.

A Pearson's correlation was run in SPSS to evaluate whether there is a relationship between the identified compounds and the measured bioactivities.
