*2.5. Mitochondrial Mass by MitoTracker Green*

The mitochondrial mass was indirectly evaluated by a fluorometric assay using MitoTracker green (MTG). After treatment, cells were washed with DMEM w/o FBS and then incubated with 0.5 μM MTG for 20 min at 37 ◦C. After that, the cells were washed two times with mammalian Ringer Krebs Medium. Micrographs were taken with a 20× objective size with numerical aperture 0.45, and zoomed in with Cytation 5 imaging, GFP filter (Ex/Em, 469 nm/525 nm).
