*4.5. Far-UV CD Measurements*

CD measurements were performed in quartz cells of 0.1 mm pathlengths using a Jasco J-810 (Jasco, Tokyo, Japan) spectropolarimeter. Far-UV CD spectra were recorded in the range of 180–260 nm with a scanning speed of 20 nm/min, bandwidth of 1 nm and integration time of 4 s. 6 scans were accumulated. Thermal denaturation was recorded in a 1 mm cell at 220 nm from 10 to 100 ◦C with scanning rate of 120 ◦C/h. The temperature was controlled using a PTE Peltier unit. The thermal denaturation profile was fitted according to the Gibbs-Helmholtz equation assuming a two-state model, which is represented by a sigmoidal curve [44]. CD spectra were quantitatively analyzed by the BeStSel method [29,30] (http://bestsel.elte.hu).
