*HOX transcript antisense RNA (HOTAIR):*

HOTAIR300 (140–440 nt) and HOTAIR440 (1–440 nt) DNA sequences cloned into pEX-A128 vector were purchased from Eurofins Genomics (Ebersberg, Germany). After 2 h digestion with EcoRV restriction enzyme at 37 ◦C, the gel-purified, linearized DNA templates were used to synthesize RNA by in-vitro transcription.
