*3.3. In Vitro p300 HAT-Mediated Acetylation Reactions*

Acetylation of wt Tau by p300 HAT was performed by combining 500 μL of 86 μM purified wt Tau (dissolved in water), 200 μL of 15 μM p300 HAT, 25 μL of 10 mM acetyl-CoA (Sigma, Saint Louis, MO, USA), and 25 μL of 1 M Tris, pH 8. The acetylation reaction was allowed to proceed for three days at RT (unless stated otherwise).
