*2.8. Number of Residues in Contact with Nucleotides in the DOT Region and in Entire Protein*

Among 1175 residues in DOT regions in our dataset, only 96 (8.17%) and 268 (22.81%) are in contact with nucleotides in the NR3.5 and the NR6 dataset, respectively. Almost all the residues have a similar tendency of binding with nucleotides in proteins, ranging between 20% to 29%, as shown in Table 3 and Table S2. However, the number of nucleotides interacting with DOT residues is somewhat different, that is, the range of interaction is 18 to 33%. The DOT residues are more likely to bind with Guanine (20.4%), followed by Cytosine and Uracil, than to binding with Adenine (13.1%).


**Table 3.** Number of interaction of nucleotides with DOT residues and with complete protein at 3.5 Å.

Percentage is mentioned in the parenthesis. Relative contact in DOT regions are calculated by *Nidt*/*Nprot* × 100.
