*4.9. ITC*

The protocol used during the ITC experiments was that previously described [46]. Briefly, the ITC experiments were carried out by using a VP-ITC instrument (Microcal, Northampton, MA, USA). Before the experiments, C-LrtA was dialysed at 4 ◦C against water at physiological pH. Dilution ITC experiments involved sequential injections of microliter amounts (10 μL) of a concentrated protein solution (498 μM, in protomer units) into the calorimetric cell (1.4 mL), which initially contained water alone.
