*2.4. Primers and Probes*

MCMV M80/80.5 forward primers (5- -CTTGCCTCAGGTGCCCTCTTATTACGGAAT-3- ) and reverse primers (5- - ATAAATCACACGTTCACTCCGTTAGTCCGG -3- ) were both synthesized by Life technologies, Camarillo, CA, USA.

MCMV *ie-1* forward primers (5- -TCAGCCATCAACTCTGCTACCAAC-3- ) and reverse primers (5- -ATCTGAAACAGCCGTATATCATCTTG-3- ) were synthesized by Life technologies, USA. TaqMan probes (5- -TTCTCTGTCAGCTAGCCAATGATATCTTCGAGC-3- ) were synthesized by Genscript, Nanjing, Jiangsu, China. The probe was labeled at the 5 end with the reporter dye FAM and at the 3- end with the quencher dye TAMRA.
