*2.7. Western Blot Analysis*

Cells were lysed using radioimmunoprecipitation assay (RIPA) buffer (sigma) containing protease inhibitor (Roche). Samples were mixed with loading buffer and heated at 95 ◦C for 10 min and then fractionated by SDS-PAGE. Proteins were transferred to a polyvinylidine fluoride membrane (Millipore) using Mini Trans-Blot Cell (Bio-Rad) and blocked with 1% bovine serum albumin. Blots were probed with relevant primary and secondary antibodies and proteins were detected by enhanced chemiluminescent reagent (Thermo Scientific).
