*2.17. Statistical Analysis*

The data were analyzed statistically using Microsoft Excel. In all cases, the values were the average of triplicate experiments and indicated as Mean ± SD (standard deviation).

Significant differences between the 2 groups in MCMV DNA copies in each organ of mice were determined using a two-tail Student's t test (type 3). Data were calculated in triplicate and expressed as Mean ± SEM (standard error of the mean). To determine real-time PCR techniques and their relative sensitivity, all real-time PCR data were calculated to DNA copies/mg of tissue. In all cases, a *p* value of ≤0.05 was considered statistically significant.
