*2.7. Quantitative RT-PCR (qRT PCR)*

Total RNA was extracted from cells, or 1 g of lung homogenate using the RNeasy Mini kit (Qiagen, Hiden, Germany) and cDNA synthesis was performed using the enzyme reverse transcriptase (TOYOBO). Next, qRT-PCR was performed using the Rotor Gene Q instrument (Qiagen, Hiden, Germany), with the QuantiTect SYBR Green PCR Master Mix (Qiagen, Hiden, Germany). The transcription level of mRNA was obtained by the 2−ΔΔ*C*<sup>t</sup> method as described previously [24] and expressed as fold induction. The RT-PCR primer sequences used as follows, RSV-G forward primer 5'-CCAAACAAACCC AATAATGATTT-3' reverse primer 5'-GCCCAGCAGGTTGGATTGT-3' Glyceraldehyde 3-phosphate dehydrogenase (GAPDH): Forward primer 5'-TGACCACAGTCCATGCCATC-3' reverse primer 5'-GACGGACACATTGGGGGTAG-3'.
