*2.5. Transcription Activator-Like E*ff*ector Nuclease (TALEN) Plasmids*

Three specific pairs (left and right) of TALEN plasmids for each targeting site MCMV1-2, 3-4, 5-6, and two nonspecific pairs W1FS-W7R1, KSHV1-2 were constructed by others. They all contain a

CMV promoter, a *Fok* I gene (cleavage domain) and a TALE DNA sequence using pTAL4 Leu (8467 bp, Addgene, Watertown, MA, USA) as the backbone vector. All ten TALEN plasmids are listed in Table 1.


**Table 1.** List of TALEN plasmids.

Abbreviations: MCMV (murine cytomegalovirus), M80/80.5 (the overlapping region of MCMV M80 and M80.5), 293T cell (human embryonic kidney 293 cells), WAS (a specific gene in 293 cells), KSHV (Kaposi's sarcoma-associated herpes virus), RTA (replication and transcription activator gene in KSHV).
