*2.4. Immunofluorescence Assay (IFA)*

Vero and C6/36 cells were infected with ZIKV or JEV-P3 at a multiplicity of infection as indicated in the results section for 1 h and the media were replaced with different concentrations of dec-RVKR-cmk or DMSO. Cells were fixed at various time points with ice-cold methanol for 10 min and then washed with PBS. Afterwards, cells were blocked with 10% bovine serum albumin (BSA) in PBS for 30 min at room temperature. Later, cells were incubated with mouse polyclonal anti-NS5 primary antibody of ZIKV or JEV for 1 h at room temperature. After washing with PBS, cells were stained with a second antibody (Alexa Fluor 488) for 30 min at room temperature. Cell nuclei were stained with 6-diamidino-2-phenyl indole (DAPI; Invitrogen). The cells were observed under fluorescence microscope (Zeiss).
