*2.2. Antibodies, Chemicals and Reagents*

The mouse monoclonal antibodies against PRRSV N protein were purchased from MEDIAN Diagnostics (Korea). Rabbit monoclonal antibodies directed against Phospho-P65, Phospho-IκBα, and mouse monoclonal antibodies directed against P65 and IκBα were purchased from Cell Signaling Technology (Beverly, MA, USA). Goat anti-rabbit IgG antibody and goat anti-mouse IgG antibody were from LI-COR Biosciences (Lincoln, NE, USA). Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) antibody was purchased from MBL Beijing Biotech (Beijing, China). Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody (Alexa Fluor 594 and 488) were purchased from Thermo Fisher Scientific (Waltham, MA, USA).

Ginsenoside Rg1 (≥98.0%) was purchased from Chengdu Biopurify Phytochemicals (Chengdu, China). LPS (lipopolysaccharide from Escherichia coli 0111:B4) was purchased from Sigma-Aldrich (MA, USA). Rg1 was dissolved in dimethylsulfoxide (DMSO, Sigma-Aldrich) and diluted with DMEM before use. The final concentration of DMSO in the cell culture medium was less than 0.4%.
