*2.12. Identification of Acteoside through HPLC*

A reversed-phase high-performance liquid chromatography (HPLC) was performed using Agilent technologies 1200 series HPLC system equipped with a DAD detector (Agilent technologies, Santa Clara, CA, USA). The binary mobile phase consisted of water containing 1% formic acid (solvent A) and acetonitrile (solvent B). All solvents were filtered through a 0.45 μm filter prior to use. The mobile phase consisted of 1% Formic acid (Solvent A) and Acetonitrile (Solvent B) in the gradient mode as follows: 0–20 min 0–40% B; 20–22 min 40–100% B; 22–25 min 100–0% B at flow rate of 1.0 mL/min at 30 ◦C.
