**4. Conclusions**

In this study, the use of a recycled diatomaceous earth as extractive phase for SPME fiber demonstrated suitable results in comparison to widely used commercial fibers. The production of the biosorbent fiber is simple and the fibers can be reused several times. The separation and detection of the analytes by GC-MS is effective and enables the determination of PAHs in accordance with current Brazilian legislation. The proposed method using the biosorbent achieved good results of parameters of merit. The method is of low cost, because the natural sorbent can be reused in numerous extractions and is widely applicable because the material is easily obtainable.

**Author Contributions:** All of the authors participated in the same proportion.

**Funding:** Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), process number 303892/2014-5. Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brasil (CAPES) —Finance Code 001.

**Acknowledgments:** The authors are grateful to the Brazilian governmental agency "Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior" for the financial support which made this research possible.

**Conflicts of Interest:** The authors declare no conflict of interest.
