*4.2. DNA Sequencing for KCNJ5, ATP1A1, ATP2B3, and CACNA1D*

DNA fragments from *KCNJ5*, *ATP1A1*, *ATP2B3*, and *CACNA1D* were amplified by PCR as previously reported [28,29]. The validity of novel mutations was confirmed by sequencing both strands of an independently amplified PCR fragment. Of the presented cohort of adrenal samples, 36 were included in the study by Fernandes-Rosa et al. [29]. Of the included samples, 16 adrenal nodules carried a mutation in the *KCNJ5* gene, six in the *ATP1A1* or *ATP2B3* genes, and five in the *CACNA1D* gene, while 19 samples had no mutations in any of these genes.
