2.4.2. Calcium Contents

To determine the calcium contents of MG-63 cells, cells were seeded at a density of 1 × 105 cells/mL on the surfaces of each Ti disc (*n* = 5) and cultured for 7 or 21 days. Ti discs were then rinsed with PBS, and treated with 0.5 N HCl for 24 h, the Ti discs containing cells were performed by centrifugation at 13,500 rpm for 1 min. Calcium contents were assessed by a QuantiChrom Calcium Assay Kit (DICA-500, BioAssay Systems, Hayward, CA, USA) using calcium chloride as a standard and a Flash Multimode Reader at 612 nm.
