*2.3. Scaffold Design and Additive Manufacturing*

SolidWorks (Waltham, Massachusetts, Estats Units) was the computer-aided design (CAD) software chosen for the scaffolds' design. The stereolithography (STL) file formats the designs that were transferred to the computer-aided manufacturing (CAM) software Slic3r to establish the fabrication parameters. This software, while maintaining the SolidWorks design, generated G-code files which can control and regulate the machine to obtain the correctly-printed scaffolds. Scaffold design features were selected based on other research work focused on tissue engineering which had similar goals to this work, i.e., cell enrichment or treatment, or drug delivery applicability. The features are described in Table 1. Previous screening experiments were carried out to adjust the range of the scaffold design features.


**Table 1.** Process parameters used for PCL scaffold printing.
